Expression of DDX25 in nuage components of mammalian spermatogenic cells: immunofluorescence and immunoelectron microscopic study

• Published in: Histochemistry and cell biology Vol. 137; no. 1; pp. 37 - 51
• Main Authors: Onohara, Yuko, Yokota, Sadaki
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.01.2012; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cellular biology; DEAD-box RNA Helicases - analysis; Developmental Biology; Enzymes; Fluorescent Antibody Technique; Guinea Pigs; Hormones; Immunology; Male; Mice; Microscopy; Microscopy, Immunoelectron; Original Paper; Rats; Rats, Wistar; Ribonucleic acid; RNA; Rodents; Spermatogonia - cytology; Spermatogonia - metabolism; Testis; Nuage; Immunoelectron microscopy; DDX25; Chromatoid body
• ISSN: 0948-6143; 1432-119X
• DOI: 10.1007/s00418-011-0875-2

The localization of DDX25/GRTH and gonadotropin-stimulated RNA helicase was studied in the spermatogenic cells of rat, mouse, and guinea pig by immunofluorescence and immunoelectron microscopy (IEM). Immunofluorescence studies identified four kinds of granular staining: (1) fine particles observed in meiotic cells; (2) small granules associated with a mitochondrial marker, appearing in pachytene spermatocytes after stage V; (3) short strands lacking the mitochondrial marker in late spermatocytes; and, (4) large irregularly shaped granules in round spermatids. IEM identified DDX25 signals in nine compartments: (1) fine dense particles in the meiotic cells; (2) intermitochondrial cement; (3) loose aggregates of 70–90 nm particles; (4) chromatoid bodies; (5) late chromatoid bodies; (6) satellite bodies; (7) granulated bodies; (8) mitochondria-associated granules; and, (9) reticulated bodies. Compartments (1) to (6) were previously classified into nuage while (7) to (9) were classified as nuage components by the present study. The results suggest that DDX25 functions in these nine compartments.