Individuality of Ag-selected and preimmune TCR repertoires

We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-s...

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Bibliographic Details
Published inImmunologic research Vol. 23; no. 1; pp. 75 - 84
Main Authors Maryanski, J L, Attuil, V, Hamrouni, A, Mutin, M, Rossi, M, Aublin, A, Bucher, P
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.01.2001
Subjects
Animals
CD8-Positive T-Lymphocytes - immunology
Computer Simulation
Flow Cytometry
Gene Library
HLA-C Antigens - immunology
Humans
Immune system
Immunology
Mice
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta - immunology
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Summary:We have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
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ISSN:0257-277X
0257-277X
1559-0755
DOI:10.1385/ir:23:1:75