Anti-inflammatory effect of Streblus asper leaf extract in rats and its modulation on inflammation-associated genes expression in RAW 264.7 macrophage cells

• Published in: Journal of ethnopharmacology Vol. 124; no. 3; pp. 566 - 570
• Main Authors: Sripanidkulchai, Bungorn, Junlatat, Jintana, Wara-aswapati, Nawarat, Hormdee, Doosadee
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 30.07.2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: animal models; Animals; Anti-inflammatory; anti-inflammatory activity; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Biological and medical sciences; Carrageenan; Carrageenan-induced edema; cell lines; Cell Survival - drug effects; cultured cells; Cyclooxygenase (COX); Cyclooxygenase 1 - biosynthesis; Cyclooxygenase 2 - biosynthesis; dosage; dose response; Edema - pathology; Edema - prevention & control; gene expression; Gene Expression - drug effects; General pharmacology; genes; Inducible nitric oxide synthase; inflammation; Inflammation - genetics; intraperitoneal injection; leaves; Lipopolysaccharides; macrophages; Macrophages - drug effects; Macrophages - metabolism; Male; Medical sciences; medicinal plants; medicinal properties; Mice; Moraceae; Moraceae - chemistry; nitric oxide synthase; Nitric Oxide Synthase Type II - antagonists & inhibitors; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant extracts; Plant Extracts - pharmacology; Plant Leaves - chemistry; prostaglandin synthase; Rats; Rats, Sprague-Dawley; RAW 264.7 macrophage; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Streblus; Streblus asper; Thailand; RAW 264.7 macrophage; NO; IC 50; IL; RNA; DMSO; DNase; MTT; COX; LPS; Streblus asper; Cyclooxygenase (COX); TNF-α; DNA; RT-PCR; SAE; Inducible nitric oxide synthase; PG; iNOS; Anti-inflammatory; Carrageenan-induced edema; Prostaglandin-endoperoxide synthase; Rat; Extract; Medicinal plant; Dicotyledones; Angiospermae; Edema; Enzyme; Pharmacognosy; Rodentia; Antiinflammatory agent; Plant leaf; Inflammation; Moraceae; Gene expression; In vitro; Nitric-oxide synthase; Vertebrata; Mammalia; Animal; Plant origin; Spermatophyta; Oxidoreductases; Carrageenan; Polysaccharide; Macrophage
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2009.04.061

Streblus asper is a medicinal plant from Thailand used in folk medicine for the treatment of several inflammatory diseases. In this study, we investigated the anti-inflammatory effect of Streblus asper leaf ethanolic extract (SAE). The experimental carrageenan-induced paw edema in rats was performed in which the SAE at doses of 125, 250, 500 mg/kg body weight was intraperitoneally administered to the rats. Then, reverse transcriptive polymerase chain reaction (RT-PCR) technique was also performed to determine the effect of SAE on the expression of inflammation-associated genes in RAW 264.7 macrophage cells stimulated with lipopolysaccharide (LPS). The SAE at all given doses caused a significant dose-dependent inhibition of edema ( p < 0.05). Moreover, the significant and dose-dependent LPS-induced cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) mRNA expressions were demonstrated in RAW 264.7 cells treated with SAE. The inhibition is selective, since COX-1 mRNA expression did not change in the presence of SAE. The results of this study are the first scientific evidence on the molecular effects of Streblus asper as a potential anti-inflammatory agent, which supports the fact that the plant is employed in traditional remedies.