Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam

African swine fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam began in 2019, posing a threat to spread to the neighbouring Asian countries. Without a commercial vaccine or efficient chemotherapeutics, rapid diagnosis and necessary biosecu...

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Published inTransboundary and emerging diseases Vol. 68; no. 4; pp. 2595 - 2602
Main Authors Tran, Diem Hong, Tran, Hau Thi, Le, Uyen Phuong, Vu, Xuan Dang, Trinh, Thi Bich Ngoc, Do, Hoang Dang Khoa, Than, Van Thai, Bui, Le Minh, Vu, Van Van, Nguyen, Thi Lan, Phung, Huong Thi Thu, Le, Van Phan
Format Journal Article
LanguageEnglish
Published Berlin Hindawi Limited 01.07.2021
Subjects
African swine fever
Animal health
Asfarviridae
Assaying
Biosecurity
colorimetric
Colorimetry
crude samples
Deoxyribonucleic acid
Diagnosis
Diagnostic systems
Disease control
DNA
Domestic animals
Equipment costs
Fever
in‐field diagnosis
LAMP
Outbreaks
Polymerase chain reaction
Portable equipment
rapid testing
Swine
Vaccines
Viral diseases
Viruses
Vietnam
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Summary:African swine fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam began in 2019, posing a threat to spread to the neighbouring Asian countries. Without a commercial vaccine or efficient chemotherapeutics, rapid diagnosis and necessary biosecurity procedures are required to control the disease. While the diagnostic method of ASF recommended by the World Organization of Animal Health is real‐time PCR, the ideal diagnosis procedure including master mix setup, template extraction and a high‐cost qPCR equipment for many samples being tested simultaneously is not portable. In this study, a colorimetric loop‐mediated isothermal amplification (LAMP) assay was modified and evaluated for ASF virus detection using crude serum samples collected from domestic pigs in Vietnam during the 2019 outbreak. The LAMP results can be readily visualized to the naked eye within 30 min without the requirement of DNA extraction and sophisticated equipment. The sensitivity, specificity and limit of detection of direct colorimetric LAMP assay were comparable to a commercial diagnostic real‐time PCR kit. Results strongly indicate that the adapted colorimetric LAMP assay has a remarkable potential for the in‐field diagnosis of ASF.
ISSN:1865-1674
1865-1682
DOI:10.1111/tbed.13879