Tunicamycin Tightens the Glucose- and D-Allose-Mediated Control of Hexose Transport in a Metabolic Fibroblast Mutant

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 84; no. 11; pp. 3678 - 3680
• Main Authors: ULLREY, D. B, KALCKAR, H. M
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.06.1987; National Acad Sciences
• Subjects: Animals; Biochemistry; Biological and medical sciences; Cell Line; Cell lines; Cell physiology; Cricetinae; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Glucose - pharmacology; Glycoproteins; hexose; Hexoses; Hexoses - metabolism; Kinetics; Membrane and intracellular transports; Metabolism; Molecular and cellular biology; Monosaccharide Transport Proteins - metabolism; Mutation; Physiological regulation; Sugars; tunicamycin; Tunicamycin - pharmacology; Ungulates; Vertebrata; Hexose; Regulation(control); Mammalia; Membrane transport; Animal; Rodentia; Established cell line; Glucose; Mutation; Fibroblast; Hamster
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.11.3678

The hexose transport system of a fibroblast mutant, DS7, unable to convert glucose 6-phosphate to fructose 6-phosphate (``the phosphoglucose isomerase mutant''), is subject to a specific down-regulation (``curb'') evoked by only glucose or D-allose. Neither fructose nor mannose has a curbing effect on this mutant. Further addition of tunicamycin intensified the transport curb on the mutant mediated by glucose or allose. Mannose added to the parental cell line 023 seems able to mimic a glucose-mediated transport curb. In this line, but not the mutant, tunicamycin also intensifies a mannose-mediated curb. It seems that the tightening of the allose-mediated curb is a function of a specific type of transport regulation and perhaps too of interference with glycosylation of the hexose transporter. Furthermore, this type of curb can be strikingly reversed by shifting the cultures to medium containing fructose.