Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt
Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead...
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Published in | Journal of basic microbiology Vol. 48; no. 3; pp. 168 - 176 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley-VCH Verlag
01.06.2008
WILEY-VCH Verlag WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu²⁺, Co²⁺, Ni²⁺, Zn²⁺, Cr³⁺, Cd²⁺and Pb²⁺ were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni²⁺ and Pb²⁺ stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
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Bibliography: | http://dx.doi.org/10.1002/jobm.200700338 ArticleID:JOBM200700338 ark:/67375/WNG-2JM9V6ST-F istex:3B6A253467BB77892F23D67CABFE6F09F777B771 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0233-111X 1521-4028 |
DOI: | 10.1002/jobm.200700338 |