Comparison of acidified glycerol lysis test, Pink test and osmotic fragility test in hereditary spherocytosis: effect of incubation

In this study we compared the results of the acidified glycerol lysis test, the Pink test and the osmotic fragility test in 38 patients with hereditary spherocytosis and in healthy controls. The sensitivity of the acidified glycerol lysis test was 81.6% when performed within 3 h after blood collecti...

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Bibliographic Details
Published inEuropean journal of haematology Vol. 40; no. 3; p. 227
Main Authors Bucx, M J, Breed, W P, Hoffmann, J J
Format Journal Article
LanguageEnglish
Published England 01.03.1988
Subjects
Adolescent
Adult
Female
Glycerol
Hemolysis
Humans
Hydrogen-Ion Concentration
Male
Middle Aged
Osmotic Fragility
Reference Values
Spherocytosis, Hereditary - blood
Spherocytosis, Hereditary - surgery
Splenectomy
Time Factors
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Summary:In this study we compared the results of the acidified glycerol lysis test, the Pink test and the osmotic fragility test in 38 patients with hereditary spherocytosis and in healthy controls. The sensitivity of the acidified glycerol lysis test was 81.6% when performed within 3 h after blood collection. After incubating for 24 h, the sensitivity increased to 100% whereas the specificity remained maximal. Similar incubation did not improve the diagnostic utility of the Pink test. All patients, but none of the controls, showed a positive osmotic fragility test. It is concluded, because of sensitivity and specificity in this study, that the acidified glycerol lysis test after incubation and the osmotic fragility test are superior to the Pink test in detecting spherocytosis.
ISSN:0902-4441
DOI:10.1111/j.1600-0609.1988.tb00828.x