SCID-repopulating activity of human umbilical cord blood-derived hematopoietic stem and/or progenitor cells in a nonobese diabetic/Shi-SCID mice serial xenotransplantation model and immune cell activities in vitro: a comparative study of the filter method and the hydroxyethyl starch method

• Published in: Transfusion (Philadelphia, Pa.) Vol. 45; no. 12; pp. 1899 - 1908
• Main Authors: Yasutake, Mikitomo, Zheng, Yizhou, Inoue-Nagamura, Tokiko, Akagawa, Eiji, Tokushima, Yasuo, Terashima, Shuji, Takahashi, Tsuneo A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK and Malden, USA Blackwell Science Inc 01.12.2005; Blackwell Publishing
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Blood Preservation - methods; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Blood. Blood coagulation. Reticuloendothelial system; Bone marrow, stem cells transplantation. Graft versus host reaction; Cord Blood Stem Cell Transplantation; Cryopreservation - methods; Female; Filtration - methods; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - immunology; Humans; Hydroxyethyl Starch Derivatives; Medical sciences; Mice; Mice, Inbred NOD; Mice, SCID; Phagocytosis - immunology; Pharmacology. Drug treatments; Severe Combined Immunodeficiency - therapy; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation, Heterologous; Endocrinopathy; Human; Transfusion; Stem cell; Diabetes mellitus; Rodentia; Hematopoietic cell; Biological activity; Vertebrata; Mammalia; Mouse; Filter; Animal; Umbilical cord; Starch(hydroxyethyl); Comparative study
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1111/j.1537-2995.2005.00598.x

BACKGROUND: A novel filter system was developed for umbilical cord blood (UCB) volume reduction. The aim of this study was to compare the functions of cryopreserved UCB cells processed by the filter and by the hydroxyethyl starch (HES) sedimentation method from the aspect of the graft quality. STUDY DESIGN AND METHODS: UCB specimens were divided into two portions, processed in parallel by the filter or HES, and then cryopreserved in the clinical setting. The thawed UCB specimens containing 1 × 105 CD34+ cells were injected into nonobese diabetic/Shi‐SCID mice, and the engraftment capacity in primary and secondary transplants was assessed. The functions of natural killer (NK) cells and monocyte‐derived dendritic cells (DCs) were also assayed in vitro. RESULTS: The percentage of recovery of CD34+ cells by both methods was equivalent. In the marrow of the primary transplant recipients, the percentage of hCD45+ cells in the filter group and HES group was 58.2 ± 31.6 and 46.5 ± 28.4 percent, respectively (p = 0.016). The engraftment capacity and multilineage differentiation in the secondary transplantations were equal in both groups. The cytotoxic activity of the NK cells and phagocytosis activity of the DCs from both the groups were similar. CONCLUSION: The filter yielded a desirable percentage of recovery of hematopoietic cells with engraftment ability in the clinical setting. Thus, it is considered that the filter system may be useful for UCB banking for cord blood transplantation.