Comparative study of human and rabbit cell infection with cell-free HTLV-I

Infection of human and rabbit cells with cell-free HTLV-I was studied by PCR analysis. Both human and rabbit PBL were infected similarly by cell-free virus of both human and rabbit cell origin. Cells were infected with the cell-free virus without prior treatment and regardless of the concentration o...

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Bibliographic Details
Published inJournal of medical virology Vol. 39; no. 1; p. 75
Main Authors Yamade, I, Isono, T, Ishiguro, T, Yoshida, Y
Format Journal Article
LanguageEnglish
Published United States 01.01.1993
Subjects
Animals
Base Sequence
Blotting, Southern
Cell Line
Cell-Free System
DNA, Viral - analysis
HTLV-I Infections - transmission
Human T-lymphotropic virus 1 - genetics
Human T-lymphotropic virus 1 - growth & development
Humans
Immunoblotting
Laboratory Infection - etiology
Microscopy, Electron
Molecular Sequence Data
Polymerase Chain Reaction
Proviruses - genetics
Proviruses - isolation & purification
Rabbits
T-Lymphocytes - microbiology
Virion - pathogenicity
Virion - ultrastructure
Virus Cultivation - adverse effects
Virus Integration
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Summary:Infection of human and rabbit cells with cell-free HTLV-I was studied by PCR analysis. Both human and rabbit PBL were infected similarly by cell-free virus of both human and rabbit cell origin. Cells were infected with the cell-free virus without prior treatment and regardless of the concentration of the culture supernatant containing the virus. Human and rabbit cell lines were also infected similarly by the cell-free virus, the proviral DNA persisting for more than two months. The culture supernatants of HTLV-I-producing cells could thus be a potential cause of laboratory infections.
ISSN:0146-6615
DOI:10.1002/jmv.1890390114