Chronic cocaine exposure impairs progenitor proliferation but spares survival and maturation of neural precursors in adult rat dentate gyrus

• Published in: The European journal of neuroscience Vol. 24; no. 2; pp. 586 - 594
• Main Authors: Domínguez-Escribà, L., Hernández-Rabaza, V., Soriano-Navarro, M., Barcia, J. A., Romero, F. J., García-Verdugo, J. M., Canales, J. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2006
• Subjects: abused drugs; Animals; Bromodeoxyuridine; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Proliferation - drug effects; Cell Shape - drug effects; Cell Shape - physiology; Cell Survival - drug effects; Cell Survival - physiology; Chronic Disease; Cocaine - adverse effects; Cocaine-Related Disorders - physiopathology; Cognition Disorders - chemically induced; Cognition Disorders - physiopathology; Dentate Gyrus - drug effects; Dentate Gyrus - pathology; Dentate Gyrus - physiopathology; Disease Models, Animal; Dopamine Uptake Inhibitors - adverse effects; Down-Regulation - drug effects; Down-Regulation - physiology; Drug Administration Schedule; hippocampus; Ki-67 Antigen - metabolism; Male; Microtubule-Associated Proteins - drug effects; Microtubule-Associated Proteins - metabolism; Mitosis - drug effects; Mitosis - physiology; Mossy Fibers, Hippocampal - drug effects; Mossy Fibers, Hippocampal - pathology; neurogenesis; Neurons - drug effects; Neurons - pathology; Neuropeptides - drug effects; Neuropeptides - metabolism; Rats; Rats, Wistar; Stem Cells - drug effects; Stem Cells - pathology
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1111/j.1460-9568.2006.04924.x

Recent observations indicate that drugs of abuse, including alcohol and opiates, impair adult neurogenesis in the hippocampus. We have studied in rats the impact of cocaine treatment (20 mg/kg, daily, i.p.) on cell proliferation, survival and maturation following short‐term (8‐day) and long‐term (24‐day) exposure. Using 5′‐bromo‐2‐deoxyuridine (BrdU) and Ki‐67 as mitotic markers at the end of the drug treatments, we found that both short‐ and long‐term cocaine exposures significantly reduced cell proliferation in the dentate gyrus (DG) of the hippocampus. By labelling mitotic cells with BrdU pulses before or during the early stages of the drug treatment, we determined that long‐term cocaine exposure did not affect the survival of newly generated cells. In register with this finding, cocaine chronic exposure did not increase the number of apoptotic cells labelled by TUNEL (terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labelling). Using doublecortin (DCX) immunocytochemistry and electron microscopy, we next examined the effects of cocaine exposure on the maturation of the neural precursors and on synaptic output to CA3. DCX immunocytochemistry showed that immature hippocampal cells of rats exposed to cocaine displayed normal arborization patterns and similar degrees of colocalization with BrdU at two different developmental stages. Moreover, cocaine did not produce significant morphological alterations of the mossy fibre projection system to stratum lucidum in the CA3 area of the hippocampus. The results presented demonstrate that chronic cocaine exposure impairs proliferation dynamics in the DG without significantly altering either the survival and growth of immature cells or the structural features of terminal projections to CA3.