Developmentally regulated expression of the PD-1 protein on the surface of double-negative(CD4–CD8–) thymocytes
PD-1, a member of the Ig superfamily, was previously isolated from an apoptosis-induced T cell hybridoma 2B4.11 by subtractive hybridization. Expression of the PD-1 mRNA is restricted to thymus in adult mice. Using an anti-PD-1 mAb (J43), we examined expression of the PD-1 protein during differentia...
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Published in | International immunology Vol. 8; no. 5; pp. 773 - 780 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.05.1996
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | PD-1, a member of the Ig superfamily, was previously isolated from an apoptosis-induced T cell hybridoma 2B4.11 by subtractive hybridization. Expression of the PD-1 mRNA is restricted to thymus in adult mice. Using an anti-PD-1 mAb (J43), we examined expression of the PD-1 protein during differentiation of thymocytes in normal adult, fetal and RAG-2-/- mice with or without anti-CD3 mAb stimulation. While PD-1 was expressed only on 3–5% of total normal thymocytes, –34% of the CD4-CD8- double-negative (DN) fraction are PD-1+ cells with two distinct expression levels (low and high). PD-1high thymocytes belonged to TCR γδ lineage cells. In the DN compartment of the TCR αβ lineage, PD-1 expression started at the low level from the CD44+CD25+ stage and the majority of thymocytes expressed PD-1 at the CD44-CD25- stage in which thymocytes express TCR β chains. The anti-CD3ε antibody administration augmented the PD-1 expression as well as the differentiation of the CD44-CD25+ DN cells into the CD44-CD25- DN stage, not only in normal mice but also in RAG-2-deficient mice. The fraction of the PD-1low cells in the CD4+CD8+ double-positive (DP) compartment was very small (>5%) but increased by stimulation with the anti-CD3 antibody, although the total number of DP cells was drastically reduced. The results show that PD-1 expression is specifically induced at the stages preceding clonal selection. |
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Bibliography: | T. Honjo ArticleID:8.5.773 ark:/67375/HXZ-7NVDMSHH-1 istex:5F6F65316EB4B5C607575F5688F331AEC84D8FB0 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0953-8178 1460-2377 |
DOI: | 10.1093/intimm/8.5.773 |