Modulatory effect of Lactobacillus acidophilus KLDS 1.0738 on intestinal short-chain fatty acids metabolism and GPR41/43 expression in β-lactoglobulin-sensitized mice

• Published in: Microbiology and immunology Vol. 63; no. 8; p. 303
• Main Authors: Wang, Jun-Juan, Zhang, Qi-Min, Ni, Wei-Wei, Zhang, Xin, Li, Ying, Li, Ai-Li, Du, Peng, Li, Chun, Yu, Su-Su
• Format: Journal Article
• Language: English
• Published: Australia 01.08.2019
• Subjects: Animals; Butyrates - metabolism; Colon - metabolism; Disease Models, Animal; Fatty Acids, Volatile - metabolism; Feces - microbiology; Female; Gastrointestinal Microbiome - physiology; Hemiterpenes; Interferon-gamma - metabolism; Interleukin-17 - blood; Interleukin-4 - blood; Interleukin-6 - blood; Intestines - microbiology; Isobutyrates - metabolism; Lactobacillus acidophilus - physiology; Lactoglobulins - adverse effects; Mice; Mice, Inbred BALB C; Milk Hypersensitivity - therapy; Milk Proteins; Pentanoic Acids - metabolism; Propionates - metabolism; Receptors, G-Protein-Coupled - metabolism; RNA, Messenger - metabolism; Spleen - metabolism; Transforming Growth Factor beta - metabolism; Lactobacillus acidophilus; β-lactoglobulin allergy; gut microbiota; G-protein-coupled receptor
• ISSN: 1348-0421
• DOI: 10.1111/1348-0421.12723

We investigated the correlation between the beneficial effect of Lactobacillus acidophilus on gut microbiota composition, metabolic activities, and reducing cow's milk protein allergy. Mice sensitized with β-lactoglobulin (β-Lg) were treated with different doses of L. acidophilus KLDS 1.0738 for 4 weeks, starting 1 week before allergen induction. The results showed that intake of L. acidophilus significantly suppressed the hypersensitivity responses, together with increased fecal microbiota diversity and short-chain fatty acids (SCFAs) concentration (including propionate, butyrate, isobutyrate, and isovalerate) when compared with the allergic group. Moreover, treatment with L. acidophilus induced the expression of SCFAs receptors, G-protein-coupled receptors 41 (GPR41) and 43 (GPR43), in the spleen and colon of the allergic mice. Further analysis revealed that the GPR41 and GPR43 messenger RNA expression both positively correlated with the serum concentrations of transforming growth factor-β and IFN-γ (p < .05), but negatively with the serum concentrations of IL-17, IL-4, and IL-6 in the L. acidophilus-treated group compared with the allergic group (p < .05). These results suggested that L. acidophilus protected against the development of allergic inflammation by improving the intestinal flora, as well as upregulating SCFAs and their receptors GPR41/43.