Association of CD14, IL1B, IL6, IL10 and TNFA functional gene polymorphisms with symptomatic dental abscesses

• Published in: International endodontic journal Vol. 40; no. 7; pp. 563 - 572
• Main Authors: De Sá, A. R., Moreira, P. R., Xavier, G. M., Sampaio, I., Kalapothakis, E., Dutra, W. O., Gomez, R. S.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.07.2007
• Subjects: Abscess - immunology; Adenine; Adult; Age Factors; Case-Control Studies; Chromosome Mapping; Cross-Sectional Studies; cytokines; Cytosine; Dentistry; Female; Gene Frequency; Genetic Predisposition to Disease - genetics; Genotype; Guanine; Humans; Interleukin-10 - genetics; Interleukin-1beta - genetics; Interleukin-6 - genetics; Lipopolysaccharide Receptors - genetics; Male; molecular biology; odontogenic abscess; Periapical Periodontitis - immunology; polymorphism; Polymorphism, Genetic - genetics; receptor CD14; Thymine; Tooth Diseases - immunology; Tooth Diseases - microbiology; Tumor Necrosis Factor-alpha - genetics
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2007.01272.x

Aim  To investigate in individuals with symptomatic dental abscesses the occurrence of functional polymorphisms within five genes involved with the immune response. The functional gene polymorphisms analysed were CD14 (−260 C/T), IL1B (+3954 C/T), IL6 (−174 G/C,), IL10 (−1082 G/A) and TNFA (−308 G/A). Methodology  Genomic DNA obtained from oral swabs from individuals with symptomatic dental abscesses and asymptomatic inflammatory periapical lesions, without previous exacerbation, was submitted to restriction fragment length polymorphism (RFLP) analyses to determine each individual genotype. The chi‐square and principal components analysis tests were used for statistical analysis. Results  A significant association was observed between the occurrence of the GG genotype or the G allele expression of the polymorphic locus−174 (G/C) of the IL6 gene, and the presence of the symptomatic dental abscesses in women and in individuals ≤35 years old. The principal components analysis suggested predominance of the symptomatic dental abscesses in individuals displaying: high‐producer IL6 genotype; intermediate and high‐producer IL1B genotypes and low‐producer TNFA genotype. Conclusions  The present study suggests that genetic factors are associated with susceptibility to develop symptomatic dental abscesses.