Evaluation of activatory and inhibitory natural killer cell receptors in non-segmental vitiligo: a flow cytometric study

• Published in: Journal of the European Academy of Dermatology and Venereology Vol. 22; no. 8; pp. 970 - 976
• Main Authors: Basak, PY, Adiloglu, AK, Koc, IG, Tas, T, Akkaya, VB
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.2008
• Subjects: Adult; autoimmunity; Case-Control Studies; Female; Flow Cytometry - methods; Humans; Male; natural killer cells; Phenotype; Receptors, Natural Killer Cell - immunology; Statistics, Nonparametric; T cells; T-Lymphocyte Subsets - immunology; vitiligo; Vitiligo - immunology
• ISSN: 0926-9959; 1468-3083
• DOI: 10.1111/j.1468-3083.2008.02681.x

Background  Recent observations established the role of altered cellular immunity and autoimmune hypothesis in the pathogenesis of vitiligo. There have been several reports discussing T‐cell and natural killer (NK) cell populations, but NK cell receptors were not evaluated in vitiligo. Objective  The purpose of this investigation was to assess the role of T and NK cells as well as activatory and inhibitory NK cell receptor alterations in the pathogenesis of vitiligo and whether any aberrations were correlated with clinical findings of the disease. Patients/methods  Fifty‐three patients with non‐segmental vitiligo and 45 age‐ and sex‐matched healthy controls were enrolled in the study. The percentages of lymphocytes, granulocytes, monocytes and CD3, CD4, CD8, CD14, CD16, CD56, CD45, CD45RA, CD54RO, CD28, CD80, CD94, CD158a, KIR3DL‐1 receptors as well as CD94, CD158a, KIR3DL‐1 receptors on CD16+ cells were detected by using flow cytometry. The patient and control groups were compared in terms of the results of flow cytometric analysis, and the results were assessed regarding the type and activity of vitiligo. Results  The percentages of CD16+CD56+, CD3+CD16+CD56+, CD8+ and CD45RO+ cells were significantly increased in vitiligo group compared with the controls. No difference was detected between the patients and control groups in percentages of CD3+, CD4+, CD3−CD16+CD56+, CD28+, CD45+, CD45RA+, CD94+, CD158a+ and KIR3DL‐1+ cells. The percentage of CD16+CD158a+ cells was significantly decreased in a randomized selected group of vitiligo patients. There were no differences in percentage expression of studied cell surface antigens between patients in the active or stable period. CD3+ cells were significantly increased in generalized form, and CD45RO+ cells were significantly increased in acral/acrofacial form when compared with the other types of vitiligo. Conclusions  These results indicate further evidence for T and NK cell abnormalities in non‐segmental vitiligo. The present data show that NK cell activation may be responsible in the pathogenesis of vitiligo in conformity with decreased inhibitory and increased activatory NK cell receptors.