Quantitative investigations on moving chelation boundary within a continuous EDTA-based sample sweeping system in capillary electrophoresis
Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion swe...
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Published in | Electrophoresis Vol. 29; no. 19; pp. 3989 - 3998 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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01.10.2008
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Abstract | Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion sweeping yet. In this paper, the model of MCB was developed from the EDTA-based sweeping. The theoretical studies were performed for boundary movements and controllable sweeping of metal ion. The theoretical studies show that the sweeping system contains the MCB boundary and chelate boundary, and the difference between the MCB and chelate boundary velocities plays a key role in the stacking efficiency of metal ion. To demonstrate the validity of the theoretical conclusion, the experiments of continuous EDTA-based sweeping were performed with CE and a home-made apparatus of a large tube. The home-made apparatus consisted of a glass tube used for the run of continuous EDTA-based sweeping and two peristaltic pumps used for the supply of solution. The experimental results were in accordance with the theoretical conclusions above. The developed theory holds evident for significance for the mechanism of controllable stacking of metal ions in EDTA-based sweeping system in CE. |
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AbstractList | Abstract
Recently, the EDTA‐based sweeping of metal ion that combines chelate formation and in‐line sample pre‐concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion sweeping yet. In this paper, the model of MCB was developed from the EDTA‐based sweeping. The theoretical studies were performed for boundary movements and controllable sweeping of metal ion. The theoretical studies show that the sweeping system contains the MCB boundary and chelate boundary, and the difference between the MCB and chelate boundary velocities plays a key role in the stacking efficiency of metal ion. To demonstrate the validity of the theoretical conclusion, the experiments of continuous EDTA‐based sweeping were performed with CE and a home‐made apparatus of a large tube. The home‐made apparatus consisted of a glass tube used for the run of continuous EDTA‐based sweeping and two peristaltic pumps used for the supply of solution. The experimental results were in accordance with the theoretical conclusions above. The developed theory holds evident for significance for the mechanism of controllable stacking of metal ions in EDTA‐based sweeping system in CE. Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion sweeping yet. In this paper, the model of MCB was developed from the EDTA-based sweeping. The theoretical studies were performed for boundary movements and controllable sweeping of metal ion. The theoretical studies show that the sweeping system contains the MCB boundary and chelate boundary, and the difference between the MCB and chelate boundary velocities plays a key role in the stacking efficiency of metal ion. To demonstrate the validity of the theoretical conclusion, the experiments of continuous EDTA-based sweeping were performed with CE and a home-made apparatus of a large tube. The home-made apparatus consisted of a glass tube used for the run of continuous EDTA-based sweeping and two peristaltic pumps used for the supply of solution. The experimental results were in accordance with the theoretical conclusions above. The developed theory holds evident for significance for the mechanism of controllable stacking of metal ions in EDTA-based sweeping system in CE. |
Author | Li, Chenjia Fan, Liuyin Zhou, Pei Deng, Zixin Zhang, Wei Cao, Chengxi |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/18958891$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.3866/PKU.WHXB19970911 10.1016/0021-9673(95)00159-K 10.3891/acta.chem.scand.52-0709 10.3891/acta.chem.scand.53-0955 10.1126/science.282.5388.465 10.1021/ac0201880 10.1016/S0021-9673(97)00097-6 10.1021/ja01162a006 10.1016/0021-9673(93)83426-S 10.3891/acta.chem.scand.02-0841 10.1016/S0021-9673(00)01075-X 10.1016/S0021-9673(97)00498-6 10.1016/S0021-9673(97)01209-0 10.1021/ac9810866 10.1021/ac990344b 10.1021/ac049470s 10.1016/0021-9673(94)85083-6 10.1016/S0378-4347(98)00063-2 10.1002/jhrc.1240201216 10.1016/S0021-9673(02)00065-1 10.1016/S0021-9673(97)00796-6 10.1021/ac034677r 10.1021/ac60285a003 10.1021/ac990566 10.1021/ja01223a025 10.1021/cr60039a001 10.1016/S0021-9673(00)00653-1 10.1021/j100700a028 10.1002/elps.200400010 10.1021/ac9711504 10.1016/S0021-9673(97)01175-8 10.1016/S0021-9673(01)01499-6 |
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Snippet | Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation... Recently, the EDTA‐based sweeping of metal ion that combines chelate formation and in‐line sample pre‐concentration was advanced. However, the moving chelation... Abstract Recently, the EDTA‐based sweeping of metal ion that combines chelate formation and in‐line sample pre‐concentration was advanced. However, the moving... |
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SubjectTerms | Algorithms Boundary Capillary electrophoresis Chelating Agents - chemistry Chelation Copper - chemistry Edetic Acid - chemistry Electrophoresis, Capillary - methods Models, Theoretical Reproducibility of Results Sensitivity and Specificity Stacking Sweeping |
Title | Quantitative investigations on moving chelation boundary within a continuous EDTA-based sample sweeping system in capillary electrophoresis |
URI | https://api.istex.fr/ark:/67375/WNG-5SXVRHJS-G/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Felps.200800051 https://www.ncbi.nlm.nih.gov/pubmed/18958891 https://search.proquest.com/docview/36072781 https://search.proquest.com/docview/69742658 |
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