Quantitative investigations on moving chelation boundary within a continuous EDTA-based sample sweeping system in capillary electrophoresis
Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion swe...
Saved in:
Published in | Electrophoresis Vol. 29; no. 19; pp. 3989 - 3998 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley-VCH Verlag
01.10.2008
WILEY-VCH Verlag WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Recently, the EDTA-based sweeping of metal ion that combines chelate formation and in-line sample pre-concentration was advanced. However, the moving chelation boundary (MCB) existing in the sweeping system has not been investigated for the quantitative illumination of the mechanism of metal ion sweeping yet. In this paper, the model of MCB was developed from the EDTA-based sweeping. The theoretical studies were performed for boundary movements and controllable sweeping of metal ion. The theoretical studies show that the sweeping system contains the MCB boundary and chelate boundary, and the difference between the MCB and chelate boundary velocities plays a key role in the stacking efficiency of metal ion. To demonstrate the validity of the theoretical conclusion, the experiments of continuous EDTA-based sweeping were performed with CE and a home-made apparatus of a large tube. The home-made apparatus consisted of a glass tube used for the run of continuous EDTA-based sweeping and two peristaltic pumps used for the supply of solution. The experimental results were in accordance with the theoretical conclusions above. The developed theory holds evident for significance for the mechanism of controllable stacking of metal ions in EDTA-based sweeping system in CE. |
---|---|
Bibliography: | http://dx.doi.org/10.1002/elps.200800051 national "863" Scientific- technological Key Program - No. 2007AA10Z401 ark:/67375/WNG-5SXVRHJS-G istex:5DDC51B2DCA37DDBDD3D556F30065DFB764D0390 NSFC - No. 20475036; No. 20675051 ArticleID:ELPS200800051 Shanghai Leading Academic Discipline Project - No. B203 Shanghai Jiao Tong University ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200800051 |