Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration

• Published in: Journal of periodontology (1970) Vol. 66; no. 2; p. 131
• Main Authors: Sigurdsson, T J, Lee, M B, Kubota, K, Turek, T J, Wozney, J M, Wikesjö, U M
• Format: Journal Article
• Language: English
• Published: United States 01.02.1995
• Subjects: Alveolar Bone Loss - drug therapy; Alveolar Bone Loss - surgery; Animals; Ankylosis; Bone Morphogenetic Proteins; Bone Regeneration - drug effects; Dental Cementum - drug effects; Dental Cementum - physiology; Dogs; Drug Evaluation, Preclinical; Growth Substances - pharmacology; Growth Substances - therapeutic use; Humans; Lactic Acid; Male; Polyglycolic Acid; Polymers - pharmacology; Polymers - therapeutic use; Proteins - pharmacology; Proteins - therapeutic use; Random Allocation; Recombinant Proteins - pharmacology; Recombinant Proteins - therapeutic use; Regeneration - drug effects; Root Resorption
• ISSN: 0022-3492
• DOI: 10.1902/jop.1995.66.2.131

This study evaluated bone and cementum regeneration following periodontal reconstructive surgery using recombinant human bone morphogenetic protein-2 (rhBMP-2) in six beagle dogs. Surgically created mandibular supraalveolar premolar tooth defects in contralateral jaw quadrants were randomly assigned to receive rhBMP-2 or control vehicle. Clinical defect height was prepared to 5 mm. rhBMP-2 was applied with synthetic bioerodable particles and autologous blood using 20 micrograms rhBMP-2 per 100 microliters implant volume. Flaps were advanced to submerge the teeth and sutured. The dogs were sacrificed 8 weeks postsurgery. Histometric recordings included defect height, height and area of alveolar bone regeneration, height of cementum regeneration, root resorption, and ankylosis. Group means, standard deviations, and P values are shown (Student t test; n = 6). Histometric defect height for rhBMP-2 and control defects was 3.7 +/- 0.3 and 3.9 +/- 0.4 mm, respectively (P = 0.446). Height of alveolar bone regeneration amounted to 3.5 +/- 0.6 and 0.8 +/- 0.6 mm for rhBMP-2 and control defects, respectively (P = 0.000). Corresponding values for bone area were 8.4 +/- 4.5 and 0.4 +/- 0.5 mm2, respectively (P = 0.006). Cementum regeneration was observed in all experimental defects (17/17) and in 15 out of 17 controls, averaging 1.6 +/- 0.6 and 0.4 +/- 0.3 mm for rhBMP-2 and control defects, respectively (P = 0.005). Small amounts of root resorption were seen in rhBMP-2 defects, whereas controls exhibited substantial resorption (0.2 +/- 0.1 and 1.1 +/- 0.3 mm, respectively; P = 0.001).