Dynamic Partnership between KCNQ1 and KCNE1 and Influence on Cardiac IKs Current Amplitude by KCNE2

• Published in: The Journal of biological chemistry Vol. 284; no. 24; pp. 16452 - 16462
• Main Authors: Jiang, Min, Xu, Xulin, Wang, Yuhong, Toyoda, Futoshi, Liu, Xian-Sheng, Zhang, Mei, Robinson, Richard B., Tseng, Gea-Ny
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.06.2009; American Society for Biochemistry and Molecular Biology
• Subjects: Action Potentials - physiology; Adenoviridae - genetics; Animals; Biotinylation; Chlorocebus aethiops; COS Cells; Gene Expression - physiology; Green Fluorescent Proteins - genetics; Guinea Pigs; Humans; Ion Channel Gating - physiology; KCNQ1 Potassium Channel - genetics; KCNQ1 Potassium Channel - physiology; Membrane Transport, Structure, Function, and Biogenesis; Myocytes, Cardiac - cytology; Myocytes, Cardiac - physiology; Oocytes - physiology; Patch-Clamp Techniques; Potassium - metabolism; Potassium Channels, Voltage-Gated - genetics; Potassium Channels, Voltage-Gated - physiology; Protein Transport - physiology; Rats; Receptors, Cell Surface - physiology; Shal Potassium Channels - physiology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M808262200

Cardiac slow delayed rectifier (IKs) channel is composed of KCNQ1 (pore-forming) and KCNE1 (auxiliary) subunits. Although KCNE1 is an obligate IKs component that confers the uniquely slow gating kinetics, KCNE2 is also expressed in human heart. In vitro experiments suggest that KCNE2 can associate with the KCNQ1-KCNE1 complex to suppress the current amplitude without altering the slow gating kinetics. Our goal here is to test the role of KCNE2 in cardiac IKs channel function. Pulse-chase experiments in COS-7 cells show that there is a KCNE1 turnover in the KCNQ1-KCNE1 complex, supporting the possibility that KCNE1 in the IKs channel complex can be substituted by KCNE2 when the latter is available. Biotinylation experiments in COS-7 cells show that although KCNE1 relies on KCNQ1 coassembly for more efficient cell surface expression, KCNE2 can independently traffic to the cell surface, thus becoming available for substituting KCNE1 in the IKs channel complex. Injecting vesicles carrying KCNE1 or KCNE2 into KCNQ1-expressing oocytes leads to KCNQ1 modulation in the same manner as KCNQ1+KCNEx (where x = 1 or 2) cRNA coinjection. Thus, free KCNEx peptides delivered to the cell membrane can associate with existing KCNQ1 channels to modulate their function. Finally, adenovirus-mediated KCNE2 expression in adult guinea pig ventricular myocytes exhibited colocalization with native KCNQ1 protein and reduces the native IKs current density. We propose that in cardiac myocytes the IKs current amplitude is under dynamic control by the availability of KCNE2 subunits in the cell membrane.