Lactobacillus mucosae and Bifidobacterium longum Synergistically Alleviate Immobilization Stress-Induced Anxiety/Depression in Mice by Suppressing Gut Dysbiosis

• Published in: Journal of microbiology and biotechnology Vol. 29; no. 9; pp. 1369 - 1374
• Main Authors: Han, Sang-Kap, Kim, Dong Hyun
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.09.2019
• Subjects: Animals; Antidepressive Agents - administration & dosage; Antidepressive Agents - pharmacology; Antidepressive Agents - therapeutic use; Anxiety - blood; Anxiety - diet therapy; Anxiety - microbiology; Bifidobacterium longum; Brain-Derived Neurotrophic Factor - metabolism; Calcium-Binding Proteins - metabolism; Colitis - microbiology; Colitis - pathology; Corticosterone - blood; Depression - blood; Depression - diet therapy; Depression - microbiology; Disease Models, Animal; Dysbiosis - microbiology; Dysbiosis - pathology; Feces - microbiology; Gastrointestinal Microbiome - drug effects; Hippocampus - drug effects; Hippocampus - metabolism; Humans; Interleukin-6 - blood; Lactobacillus; Lipopolysaccharides - metabolism; Male; Mice, Inbred C57BL; Microfilament Proteins - metabolism; NF-kappa B - metabolism; Probiotics - administration & dosage; Probiotics - pharmacology; Probiotics - therapeutic use; Stress, Psychological - blood; Stress, Psychological - microbiology; Stress, Psychological - psychology; Tumor Necrosis Factor-alpha - blood; 생물학; anxiety; Bifidobacterium longum; Lactobacillus mucosae; depression; gut microbiota
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1907.07044

We isolated NK41 and NK46 from human feces, which induced BDNF expression in corticosterone-stimulated SH-SY5Y cells, and examined their anti-depressive effects in mice. NK41, NK46, and their (1:1) mixture significantly mitigated immobilization stress (IS)-induced anxiety-like/depressive behaviors, hippocampal NF-κB activation, BDNF expression, Iba1 cell population, and blood corticosterone, TNF-α, IL- 6, and lipopolysaccharide levels. Furthermore, they inhibited colitis marker NF-κB activation, and TNF-α expression in mice with IS-induced anxiety/depression. They additionally suppressed gut Proteobacteria and Bacteroidetes populations and bacterial lipopolysaccharide production. These findings suggest that NK41 and NK46 may alleviate anxiety/depression and colitis by suppressing gut dysbiosis.