MSCs Become Collagen-Type I Producing Cells with Different Phenotype in Allogeneic and Syngeneic Bone Marrow Transplantation

• Published in: International journal of molecular sciences Vol. 22; no. 9; p. 4895
• Main Authors: Rusch, Robert Maximilian, Ogawa, Yoko, Sato, Shinri, Morikawa, Satoru, Inagaki, Emi, Shimizu, Eisuke, Tsubota, Kazuo, Shimmura, Shigeto
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.05.2021; MDPI
• Subjects: Animals; Antigens; Bone marrow; Bone Marrow Transplantation; Collagen; Collagen (type I); Collagen Type I - biosynthesis; Eye diseases; Fibrosis; Genotype & phenotype; graft-versus-host disease (GVHD); Inflammation; Lacrimal Apparatus - cytology; Lacrimal gland; mesenchymal stem cells (MSCs); Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Pathology; Phenotype; Phenotypes; Stem cell transplantation; Stem cells; Stroma; Syngeneic grafts; Therapeutic applications; Tissue engineering; Transgenic animals; Transgenic mice; Transplantation; Transplantation, Homologous; Transplantation, Isogeneic; mesenchymal stem cells (MSCs); lacrimal gland; graft-versus-host disease (GVHD)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22094895

Mesenchymal stem cells (MSCs) have been widely used in therapeutic applications for many decades. However, more and more evidence suggests that factors such as the site of origin and pre-implantation treatment have a crucial impact on the result. This study investigates the role of freshly isolated MSCs in the lacrimal gland after allogeneic transplantation. For this purpose, MSCs from transgenic GFP mice were isolated and transplanted into allogeneic and syngeneic recipients. While the syngeneic MSCs maintained a spherical shape, allogeneic MSCs engrafted into the tissue as spindle-shaped cells in the interstitial stroma. Furthermore, the MSCs produced collagen type I in more than 85% to 95% of the detected GFP MSCs in the recipients of both models, supposedly contributing to pathogenic fibrosis in allogeneic recipients compared to syngeneic models. These findings indicate that allogeneic MSCs act completely differently from syngeneic MSCs, highlighting the importance of understanding the exact mechanisms behind MSCs.