Antioxidant and Cholinesterase Inhibitory Activity of a New Peptide From Ziziphus jujuba Fruits

• Published in: American journal of Alzheimer's disease and other dementias Vol. 28; no. 7; pp. 702 - 709
• Main Authors: Zare-Zardini, Hadi, Tolueinia, Behnaz, Hashemi, Azam, ebrahimi, Leila, Fesahat, Farzaneh
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.11.2013
• Subjects: Alzheimer Disease - drug therapy; Alzheimer Disease - enzymology; Amino Acid Sequence; Antioxidants - pharmacology; Cholinesterase Inhibitors - pharmacology; Current Topics in Research; Fruit; Molecular Sequence Data; Peptides - pharmacology; Plant Preparations - pharmacology; Ziziphus - chemistry; acetylcholinesterase; Ziziphus jujuba fruits; antioxidant; butyrylcholinesterase; Alzheimer’s disease
• ISSN: 1533-3175; 1938-2731
• DOI: 10.1177/1533317513500839

Antioxidant agents and cholinesterase inhibitors are the foremost drugs for the treatment of Alzheimer’s disease (AD). In this study, a new peptide from Ziziphus jujuba fruits was investigated for its inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes as well as antioxidant activity. This peptide was introduced as a new peptide and named Snakin-Z. The Snakin-Z displayed considerable cholinesterase inhibition against AChE and BChE. The half maximal inhibitory concentration (IC50) values of Snakin-Z against AChE and BChE are 0.58 ± 0.08 and 0.72 ± 0.085 mg/mL, respectively. This peptide has 80% enzyme inhibitory activity on AChE and BChE at 1.5 mg/mL. The Snakin-Z also had the high antioxidant activity (IC50 = 0.75 ± 0.09 mg/mL). Thus, it is suggested that Snakin-Z may be beneficial in the treatment of AD. However, more detailed researches are still required as in vivo testing its anticholinesterase and antioxidant activities.