Plantaricin LD1: A Bacteriocin Produced by Food Isolate of Lactobacillus plantarum LD1

• Published in: Applied biochemistry and biotechnology Vol. 172; no. 7; pp. 3354 - 3362
• Main Authors: Gupta, Aabha, Tiwari, Santosh Kumar
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.04.2014; Springer; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacteria; Bacteriocins - biosynthesis; Bacteriocins - chemistry; Bacteriocins - pharmacology; Bioassays; Biochemistry; Biological and medical sciences; Biotechnology; Chemistry; Chemistry and Materials Science; Detergents; E coli; Enterobacter cloacae; Enterococcus faecium; Escherichia coli; Food Microbiology; Food safety; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; High temperature; Hydrogen-Ion Concentration; Lactobacillus curvatus; Lactobacillus plantarum; Lactobacillus plantarum - growth & development; Lactobacillus plantarum - isolation & purification; Lactobacillus plantarum - metabolism; Lactococcus lactis; Micrococcus luteus; Molecular Weight; Organic solvents; Pseudomonas aeruginosa; Salmonella typhi; Shigella flexneri; Staphylococcus aureus; Vibrio; Characterization; LD1; Host range; Bacteriocin; Mode of action; Lactic acid bacteria; Lactobacillus plantarum; Lactobacillus plantarum LD1; Bacteria; Lactobacillaceae; Isolate; Foodstuff; Mechanism of action
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-014-0775-8

Plantaricin LD1, a bacteriocin produced by Lactobacillus plantarum LD1, was characterized for biochemical and antimicrobial properties. Bacteriocin showed stability at high temperatures (100 °C for 20 min and 121 °C for 15 min under 15 psi pressure), in a pH range of 2.0–8.0 and also in the presence of organic solvents, surfactants and detergents. The crude preparation was not affected by catalase, amylase and lipase but activity was reduced in the presence of pepsin, trypsin and proteinase K showing proteinaceous nature of the compound. The molecular weight of bacteriocin was found to be ∼6.5 kDa, and antimicrobial activity was confirmed by bioassay. It inhibited not only related strains but also other Gram-positive and Gram-negative bacteria such as Lactobacillus curvatus NRRL B-4562, Lactococcus lactis subsp. lactis NRRL B-1821, Enterococcus faecium NRRL B-2354, Enterobacter cloacae NRRL B-14298, Micrococcus luteus , Staphylococcus aureus , urogenic Escherichia coli , Pseudomonas aeruginosa , Salmonella typhi , Shigella flexneri and Vibrio sp. These properties of plantaricin LD1 suggest its applications not only in food safety but in therapeutics as well.