Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations

• Published in: The Korean journal of physiology & pharmacology Vol. 16; no. 3; pp. 205 - 209
• Main Authors: Kim, Seong Yeon, Kim, Dongju, Kim, Yeongseok, Kim, Hyeong Min, Hwang, Sung-Ha, Kwak, Jiyeon, Kuh, Hyo-Jeong, Lee, Jaehwi
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Physiological Society and The Korean Society of Pharmacology 01.06.2012; 대한약리학회
• Subjects: Original; 약리학; Oxidation; Skin; Hydrogen peroxide; Genistein
• ISSN: 1226-4512; 2093-3827
• DOI: 10.4196/kjpp.2012.16.3.205

The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe(3+)) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.