Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand

A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocys...

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Published inThe American journal of tropical medicine and hygiene Vol. 88; no. 2; pp. 352 - 358
Main Authors Thathaisong, Umaporn, Siripattanapipong, Suradej, Mungthin, Mathirut, Pipatsatitpong, Duangnate, Tan-ariya, Peerapan, Naaglor, Tawee, Leelayoova, Saovanee
Format Journal Article
LanguageEnglish
Published United States The American Society of Tropical Medicine and Hygiene 01.02.2013
Subjects
Adolescent
Blastocystis
Blastocystis - classification
Blastocystis - genetics
Blastocystis - isolation & purification
Blastocystis Infections - epidemiology
Blastocystis Infections - parasitology
Child
Child, Preschool
Cross-Sectional Studies
Drinking Water - parasitology
Family Characteristics
Feces - parasitology
Female
Genotype
Humans
Phylogeny
Polymerase Chain Reaction
Risk Factors
RNA, Protozoan - genetics
RNA, Protozoan - isolation & purification
RNA, Ribosomal - genetics
RNA, Ribosomal - isolation & purification
Sequence Analysis, DNA
Surveys and Questionnaires
Thailand
Young Adult
Thailand
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Summary:A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2012.12-0237