Gut microbiota regulation of T lymphocyte subsets during systemic lupus erythematosus

• Published in: BMC immunology Vol. 25; no. 1; pp. 41 - 9
• Main Authors: Lian, Fen-Ping, Zhang, Fen, Zhao, Chun-Miao, Wang, Xu-Xia, Bu, Yu-Jie, Cen, Xing, Zhao, Gui-Fang, Zhang, Sheng-Xiao, Chen, Jun-Wei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.07.2024; BioMed Central; BMC
• Subjects: Adult; Analysis; Anti-inflammatory agents; Antibiotics; Autoantibodies; Autoimmune diseases; Bacteria; Care and treatment; CD4 antigen; Cell differentiation; Composition; Correlation analysis; Diagnosis; Digestive system; Female; Flow cytometry; Gastrointestinal Microbiome - immunology; Gastrointestinal tract; Genetic testing; Gut microbiota; Helper cells; Humans; Intestinal microflora; Lupus; Lupus Erythematosus, Systemic - immunology; Lupus Erythematosus, Systemic - microbiology; Lymphocytes; Lymphocytes T; Male; Megamonas; Microbiota; Microbiota (Symbiotic organisms); Middle Aged; Normal distribution; Pathogenesis; Patients; rRNA; Statistical analysis; Systemic lupus erythematosus; T cells; T lymphocyte subsets; T-Lymphocyte Subsets - immunology; T-Lymphocytes, Regulatory - immunology; Testing; Th17 Cells - immunology; Young Adult; China; Gut microbiota; T lymphocyte subsets; Systemic lupus erythematosus
• ISSN: 1471-2172; 1471-2172
• DOI: 10.1186/s12865-024-00632-0

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by disturbance of pro-inflammatory and anti-inflammatory lymphocytes. Growing evidence shown that gut microbiota participated in the occurrence and development of SLE by affecting the differentiation and function of intestinal immune cells. The purpose of this study was to investigate the changes of gut microbiota in SLE and judge its associations with peripheral T lymphocytes. A total of 19 SLE patients and 16 HCs were enrolled in this study. Flow cytometry was used to detect the number of peripheral T lymphocyte subsets, and 16 s rRNA was used to detect the relative abundance of gut microbiota. Analyzed the correlation between gut microbiota with SLEDAI, ESR, ds-DNA and complement. SPSS26.0 software was used to analyze the experimental data. Mann-Whitney U test was applied to compare T lymphocyte subsets. Spearman analysis was used for calculating correlation. Compared with HCs, the proportions of Tregs (P = 0.001), Tfh cells (P = 0.018) and Naïve CD4 + T cells (P = 0.004) significantly decreased in SLE patients, and proportions of Th17 cells (P = 0.020) and γδT cells (P = 0.018) increased in SLE. The diversity of SLE patients were significantly decreased. Addition, there were 11 species of flora were discovered to be distinctly different in SLE group (P < 0.05). In the correlation analysis of SLE, Tregs were positively correlated with Ruminococcus2 (P = 0.042), Th17 cells were positively correlated with Megamonas (P = 0.009), γδT cells were positively correlated with Megamonas (P = 0.003) and Streptococcus (P = 0.004), Tfh cells were positively correlated with Bacteroides (P = 0.040), and Th1 cells were negatively correlated with Bifidobacterium (P = 0.005). As for clinical indicators, the level of Tregs was negatively correlated with ESR (P = 0.031), but not with C3 and C4, and the remaining cells were not significantly correlated with ESR, C3 and C4. Gut microbiota and T lymphocyte subsets of SLE changed and related to each other, which may break the immune balance and affect the occurrence and development of SLE. Therefore, it is necessary to pay attention to the changes of gut microbiota and provide new ideas for the treatment of SLE.