Autophagy Prevents Oxidative Stress-Induced Loss of Self-Renewal Capacity and Stemness in Human Tendon Stem Cells by Reducing ROS Accumulation

• Published in: Cellular physiology and biochemistry Vol. 39; no. 6; pp. 2227 - 2238
• Main Authors: Chen, Hua, Ge, Heng-an, Wu, Gen-bing, Cheng, Biao, Lu, Yong, Jiang, Chaoyin
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Cell Physiol Biochem Press GmbH & Co KG 01.01.2016
• Subjects: Adult; Autophagy; Autophagy - drug effects; Cell Differentiation - drug effects; Cell Self Renewal - drug effects; Humans; Hydrogen Peroxide - pharmacology; Original Paper; Oxidative stress; Oxidative Stress - drug effects; Protective Agents - pharmacology; Reactive Oxygen Species - metabolism; Self-renewal; Sirolimus - pharmacology; Stem Cells - drug effects; Stem Cells - metabolism; Stem Cells - pathology; Stemness; Tendon stem cells; Tendons - pathology; Oxidative stress; Stemness; Self-renewal; Autophagy; Tendon stem cells
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000447916

Background/Aims: Tendon stem cells (TSCs) exhibit a high self-renewal capacity, multi-differentiation potential, and low immunogenicity; thus, these cells might provide a new cell source for tendon repair and regeneration. TSCs are exposed to increased oxidative stress at tendon injury sites; however, how TSCs maintain their stemness under oxidative stress is not clear. Methods and Results: In this study, we found that H 2 O 2 treatment increased ROS accumulation in human TSCs (hTSCs) and resulted in loss of self-renewal capacity and stemness, as reflected in reduced colony formation and proliferation, decreased expression of the stemness markers Nanog, Oct-4, NS, and SSEA-4, and impaired differentiation capability. These H 2 O 2 -induced damages were prevented by pretreatment with starvation or rapamycin. Pretreatment with starvation or rapamycin prior to H 2 O 2 exposure also led to decreased intracellular and mitochondrial ROS accumulation along with increased autophagic activity, as manifested in increased LC3 cleavage, Beclin-1 expression, and GFP-LC3-labeled autophagosome formation. Autophagy inhibition by 3-MA or CQ, or by shRNA silencing of Agt-7 or Beclin-1 reduced the protective effects of starvation and rapamycin on H 2 O 2 -treated hTSCs. Conclusion: Thus, the findings of this study suggest that autophagy prevents oxidative stress-induced loss of self-renewal capacity and stemness in hTSCs through suppression of ROS accumulation.