Biological and Genetic Characteristics of Tumor-Initiating Cells in Colon Cancer

• Published in: Annals of surgical oncology Vol. 15; no. 2; pp. 638 - 648
• Main Authors: Ieta, Keisuke, Tanaka, Fumiaki, Haraguchi, Naotsugu, Kita, Yoshiaki, Sakashita, Hiroyuki, Mimori, Koshi, Matsumoto, Toshifumi, Inoue, Hiroshi, Kuwano, Hiroyuki, Mori, Masaki
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.02.2008; Springer Nature B.V
• Subjects: AC133 Antigen; Animals; Antigens, CD - genetics; Antigens, CD - physiology; Cell Proliferation; Colonic Neoplasms - genetics; Colonic Neoplasms - physiopathology; Flow Cytometry; Gastrointestinal Oncology; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - physiology; Glycoproteins - genetics; Glycoproteins - physiology; Humans; Immunohistochemistry; In Vitro Techniques; Medicine; Medicine & Public Health; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplastic Stem Cells - physiology; Oligonucleotide Array Sequence Analysis; Oncology; Peptides - genetics; Peptides - physiology; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells; Surgery; Surgical Oncology; Tumor-initiating cells; Colon cancer; Stem cells
• ISSN: 1068-9265; 1534-4681
• DOI: 10.1245/s10434-007-9605-3

Background Human prominin-1 (PROM1, CD133) was used as a marker to detect stem cells (progenitor cells) and cancer stem cells (tumor-initiating cells) in various tissues. The purpose of this study was to investigate the biological and genetic characteristics of tumor-initiating cells in colon cancer with both in vitro and in vivo analyses. Methods The CD133 expression of 12 colon cancer cell lines was evaluated. CD133 + cells were isolated by flow cytometry and examined for in vivo tumor formation, in vitro proliferation, colony formation, and invasion ability. Additionally, we used microarray analysis to compare gene expression profiles between CD133 + and CD133 – isolated cells. Results CD133 + cells were found in 5 of 12 colon cancer cell lines. Isolated CD133 + cells from the HT29 colon cancer cell line exhibited a higher tumorigenic potential than CD133 – cells in the in vivo tumor formation assay. Furthermore, it was shown that CD133 + cells are more proliferative and have higher colony-forming and invasive abilities than CD133 – cells in vitro . Microarray analysis found differential gene expression correlating with CD133 expression. Conclusions It was confirmed that CD133 + cells in colon cancer are useful markers for the detection of tumor-initiating cells. Intimate biological and genetic features of CD133 + cells in colon cancer cell lines were also revealed. The biological characteristics of CD133 + cells and differentially expressed genes in these cells will help elucidate more details of tumor-initiating cells in colon cancer.