Electrochemical properties of cytochroms P450 using nanostructured electrodes: Direct electron transfer and electro catalysis

• Published in: Journal of inorganic biochemistry Vol. 101; no. 5; pp. 859 - 865
• Main Authors: Shumyantseva, V.V., Bulko, T.V., Rudakov, Yu.O., Kuznetsova, G.P., Samenkova, N.F., Lisitsa, A.V., Karuzina, I.I., Archakov, A.I.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2007
• Subjects: Animals; Aryl Hydrocarbon Hydroxylases - chemistry; Benzphetamine - chemistry; Biosensing Techniques - methods; Catalysis; Cytochrome P-450 CYP1A2 - chemistry; Cytochrome P-450 Enzyme System - chemistry; Cytochrome P450; Cytochrome P450 Family 2; Gold; Gold nanoparticles; Ketoconazole; Ketoconazole - chemistry; Lanosterol; Lanosterol - chemistry; Metal Nanoparticles - chemistry; Nanostructured electrodes; Oxidation-Reduction; Oxidoreductases - chemistry; Potentiometry; Quaternary Ammonium Compounds - chemistry; Rabbits; Sterol 14-Demethylase; Lanosterol; Ketoconazole; Cytochrome P450; Gold nanoparticles; Nanostructured electrodes
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/j.jinorgbio.2007.01.015

The present study demonstrates direct electron transfer between cytochromes P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14α-demethylase (CYP51b1) on the one hand and screen-printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB) on the other. Electro detection of heme proteins was possible when 2–200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, and lanosterol) and with P450 inhibitor (ketoconazole) were analyzed using cyclic voltammetry (CV), square wave voltammetry (SWV) differential pulse voltammetry (DPV), and amperometry.