Autoantibodies to nuclear envelope antigens in chronic fatigue syndrome

We have identified and partially characterized the autoantibodies in sera of 60 patients with chronic fatigue syndrome. Approximately 52% of the sera were found to react with nuclear envelope antigens. The combination of nuclear rim staining observed in immunofluorescence microscopy and immunoblot a...

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Bibliographic Details
Published inThe Journal of clinical investigation Vol. 98; no. 8; pp. 1888 - 1896
Main Authors Konstantinov, K, von Mikecz, A, Buchwald, D, Jones, J, Gerace, L, Tan, E M
Format Journal Article
LanguageEnglish
Published United States 15.10.1996
Subjects
Adult
Antibodies, Antinuclear - blood
Autoantibodies - blood
Fatigue Syndrome, Chronic - immunology
Female
Humans
Immunoblotting
Lamin Type B
Lamins
Male
Middle Aged
Nuclear Envelope - immunology
Nuclear Proteins - analysis
Vimentin - immunology
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Summary:We have identified and partially characterized the autoantibodies in sera of 60 patients with chronic fatigue syndrome. Approximately 52% of the sera were found to react with nuclear envelope antigens. The combination of nuclear rim staining observed in immunofluorescence microscopy and immunoblot analysis of highly purified nuclear envelope proteins provided initial characterization of these autoantibodies. Further characterization showed that some sera immunoprecipitated the in vitro transcription and translation product of a human cDNA clone encoding the nuclear envelope protein lamin B1. The autoantibodies were of the IgG isotype. The occurrence of autoantibodies to a conserved intracellular protein like lamin B1 provides new laboratory evidence for an autoimmune component in chronic fatigue syndrome.
Bibliography:ObjectType-Article-1
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ISSN:0021-9738
1558-8238
DOI:10.1172/jci118990