Establishment and Characterization of Cell Lines, MeMS and MeFS, Derived from Spontaneous Rat Mesotheliomas

• Published in: Journal of Veterinary Medical Science Vol. 58; no. 7; pp. 693 - 697
• Main Authors: SHIBUYA, Kazumoto, SHIBUYA, Nobuko, NUNOYA, Tetsuo, YAMATE, Jyoji, SAITOH, Toshiki, KIZAKI, Hideyuki, TAJIMA, Masanori
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.1996; Japan Science and Technology Agency
• Subjects: Aging; Animals; Cell Line; Culture Techniques - methods; Female; Male; mesothelioma; Mesothelioma - pathology; Mesothelioma - ultrastructure; Mesothelioma - veterinary; Neoplasm Transplantation; Organelles - pathology; Organelles - ultrastructure; rat; Rats; Rats, Inbred F344; Rodent Diseases; Transplantation, Isogeneic
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.58.693

New cell lines, designated MeMS and MeFS, were established from the epithelial type of mesotheliomas occurring spontaneously in aged male and female Fischer 344 rats, respectively. Morphologically, MeMS and MeFS expressed the epithelial phenotype. Coexpression of keratin and vimentin in both cell lines was confirmed by the immunocytochemistry, one-dimensional gel electrophoresis and immunoblotting for intermediate filament proteins. Hyaluronic acid synthesis of both MeMS and MeFS was demonstrated by the analysis using high-performance liquid chromatography. MeMS and MeFS seem to be useful models for studying the biological nature of rat mesotheliomas.