Ecotoxicity Bioassays on Leachates from Poultry Manure

• Published in: Bulletin of environmental contamination and toxicology Vol. 90; no. 4; pp. 401 - 404
• Main Authors: Delgado, M., de Imperial, R. Miralles, Alonso, F., Rodríguez, C., Martín, J. V.
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.04.2013; Springer; Springer Nature B.V
• Subjects: Acute toxicity; Algae; Animal, plant and microbial ecology; Animals; Applied ecology; Aquatic Pollution; Bioassays; Biological and medical sciences; Biological Assay; Cell Line; Chlorella vulgaris; Chlorella vulgaris - drug effects; Crustaceans; Daphnia - drug effects; Daphnia magna; Earth and Environmental Science; Ecotoxicology; Ecotoxicology, biological effects of pollution; Electric Conductivity; Environment; Environmental Chemistry; Environmental Health; Fishes; Freshwater; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Landfills; Leachates; Manure; Manures; Mortality; Nitrates - analysis; Pollution; Poultry; Poultry manure; Sawdust; Soil Science & Conservation; Techniques; Toxicity; Toxicity Tests; Waste disposal sites; Waste Water Technology; Water Management; Water Pollution Control; Poultry manure; In vitro RTG-21; Bioassay; Ecotoxicology; Toxicity; Algae; Chlorella vulgaris; Cladocera; Chlorophyceae; In vitro; Crustacea; Vertebrata; Cell line; Chlorophyta; Arthropoda; Pisces; Agricultural waste; Invertebrata; Branchiopoda; Animal waste; Leachate; Daphnia magna
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-012-0936-9

The main aim of this study was to evaluate the toxicity of different poultry manure landfill leachates, using a well-known toxicity test system (MS3). The bioassay was made using a battery of toxicity tests including acute toxicity with crustacean ( Daphnia magna ), algae ( Chlorella vulgaris ) and the in vitro toxicity test with the fish cell line RTG-2. On D. magna was high mortality for zero time and almost 100 % and 70 %–80 % mortality for sawdust and straw poultry manure respectively. No effects on C. vulgaris , was observed after the leachate exposure. None of the parameters considered: protein, EROD activity, β-gal activity and neutral red, showed differences between control test and the leachate collected from exposure to poultry manure.