Period of Hyperbaric Oxygen Delivery Leads to Different Degrees of Hepatic Ischemia/Reperfusion Injury in Rats

• Published in: Transplantation proceedings Vol. 48; no. 2; pp. 516 - 520
• Main Authors: Chaves, J.C, Neto, F.S, Ikejiri, A.T, Bertoletto, P.R, Teruya, R, Santos Simões, R, Tikazawa, E.H, Liu, J.B, Carrara, F.L, Taha, M.O, Fagundes, D.J
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2016
• Subjects: Animals; Disease Models, Animal; Hyperbaric Oxygenation - methods; Ischemic Preconditioning; Liver - blood supply; Liver - pathology; Liver Diseases - metabolism; Liver Diseases - pathology; Liver Diseases - therapy; Male; Oxygen - metabolism; Rats; Rats, Wistar; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - therapy; Surgery
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2015.11.035

Abstract Background This study evaluated the morphology of the rat liver when hyperbaric oxygen (HBO) was used at various stages of ischemia and reperfusion. Methods Thirty-two male Wistar rats, subjected to 30 minutes of hepatic ischemia and 30 minutes of reperfusion, were randomly assigned as follows: GIR (n = 8), control without HBO; GHBO/I (n = 8), in which HBO was applied only during ischemia; GHBO/R (n = 8), HBO only during reperfusion; and GHBO/IR (n = 8), HBO during both ischemia and reperfusion. Feasibility scores of hepatocytes were determined by assessing 8 items related to liver injury. Results The histologic injury score of the hepatic specimens was significantly lower in the GHBO/I group (79.0 ± 0.1) compared with the GIR group (135.0 ± 0.1). HBO was not effective when applied during reperfusion (GHBO/R, 151.3 ± 0.1) or during the ischemia plus reperfusion period (GHBO/IR, 131.0 ± 0.1). The sum was significantly higher ( P  < .05) in HBO-treated animals during the reperfusion period (ie, in the GHBO/R group compared with any of the other groups). Conclusions A favorable effect was obtained when HBO was administered early during ischemia. HBO given in later periods of reperfusion was associated with a more severe sum index percentage of liver damage.