Treatment of estrogens and androgens in dairy wastewater by a constructed wetland system

• Published in: Water research (Oxford) Vol. 46; no. 7; pp. 2333 - 2343
• Main Authors: Cai, Kai, Elliott, Christopher T., Phillips, Debra H., Scippo, Marie-Louise, Muller, Marc, Connolly, Lisa
• Format: Journal Article Web Resource
• Language: English
• Published: Kidlington Elsevier Ltd 01.05.2012; Elsevier; Pergamon Press
• Subjects: Androgen; Applied sciences; Dairy wastewater; Dairying; Environmental sciences & ecology; Estrogen; Exact sciences and technology; Filtration; Genes, Reporter - genetics; Hormones - chemistry; Hormones - genetics; Life sciences; Pollution; Reporter gene assay; Sciences de l’environnement & écologie; Sciences du vivant; Solid Phase Extraction; Treatment system; Waste Disposal, Fluid - methods; Water Pollutants, Chemical - chemistry; Water Purification - methods; Water treatment and pollution; Wetlands; Reporter gene assay; Dairy wastewater; Treatment system; Androgen; Estrogen; Solid phase extraction; Microbiology; Toxicity; 17β-Estradiol; Waste water; Biological purification; Health and environment; Decontamination; Pathogenic; Biological contamination; Wetland; Public health; Organic compounds; Filtration; Endocrine disruptor; Testosterone; Artificial medium; Surface water; Toxic equivalency factor; Waste water purification; Sex steroid hormone
• ISSN: 0043-1354; 1879-2448; 1879-2448
• DOI: 10.1016/j.watres.2012.01.056

Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens, there is limited data on the presence of hormones in this type of treatment system. The objective of this study was to evaluate the ability of the CWS to reduce estrogenic and androgenic hormone concentration in dairy wastewater. This was achieved through a year long study on dairy wastewater samples obtained from a surface flow CWS. Analysis of hormonal levels was performed using a solid phase extraction (SPE) sample clean-up method, combined with reporter gene assays (RGAs) which incorporate relevant receptors capable of measuring total estrogenic or androgenic concentrations as low as 0.24 ng L−1 and 6.9 ng L−1 respectively. Monthly analysis showed a mean removal efficiency for estrogens of 95.2%, corresponding to an average residual concentration of 3.2 ng L−1 17β-estradiol equivalent (EEQ), below the proposed lowest observable effect concentration (LOEC) of 10 ng L−1. However, for one month a peak EEQ concentration of 115 ng L−1 was only reduced to 18.8 ng L−1. The mean androgenic activity peaked at 360 ng L−1 and a removal efficiency of 92.1% left an average residual concentration of 32.3 ng L−1 testosterone equivalent (TEQ). The results obtained demonstrate that this type of CWS is an efficient system for the treatment of hormones in dairy wastewater. However, additional design improvements may be required to further enhance removal efficiency of peak hormone concentrations. [Display omitted] ► Endocrine disruptors are a serious concern for the health and safety of aquatic resources. ► Estrogenic and androgenic hormonal activity is assessed in dairy farm wastewaters. ► Activity up to 725.3 ng L−1 was measured in a Constructed wetland system (CWS). ► CWS reduced estrogens by 83.7%–98.6% and androgens by 84.9%–96.2%.