Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats

• Published in: Cellular physiology and biochemistry Vol. 40; no. 6; pp. 1354 - 1366
• Main Authors: Chu, Shuang, Wang, Li, Mao, Xiao-dong, Peng, Wen
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Cell Physiol Biochem Press GmbH & Co KG 01.01.2016
• Subjects: Animals; Aorta - drug effects; Aorta - physiopathology; Body Weight - drug effects; Chronic kidney disease; Drugs, Chinese Herbal - pharmacology; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiopathology; Huangqi Decoction; Kidney - pathology; Kidney - physiopathology; Kidney - surgery; Male; NADPH Oxidases - metabolism; Nephrectomy; Nitric oxide; Nitric Oxide - metabolism; Original Paper; Oxidative stress; Rats, Wistar; Reactive Oxygen Species - metabolism; Vascular dysfunction; Huangqi Decoction; Oxidative stress; Chronic kidney disease; Vascular dysfunction; Nitric oxide
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000453188

Background/Aims: Endothelial dysfunction is a major factor in the progression of chronic kidney disease, which correlates with oxidative stress and NO deficiency. Huangqi decoction (HQD) is a potential anti-oxidant ingredient in renoprotection. However, the underlying mechanisms remained identified. Therefore, we investigated whether HQD exhibit improvement in endothelial dysfunction in the 5/6 nephrectomy (Nx) rat model. Methods: Male Wistar rats (180 - 250 g) were divided into sham, Nx and Nx + HQD (0.05, 0.15 and 0.45 g/kg) group, respectively. Renal function and histology were examined with ELISA and Immunohistochemical analysis. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Oxidative stress and NO bioavailability were detected by ELISA, DHE-staining, DAF-2 staining and western blotting. Results: Compared with Nx rats, HQD treatment reversed the functional and structural changes of kidney significantly. Besides, endothelium-dependent relaxation of rat aortas was also improved by HQD treatment. NADPH oxidase and ROS generation were inhibited while NO bioavailability was enhanced. Conclusion: HQD can act as a potent prescription for the treatment of endothelium related vascular complications.