Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo

• Published in: World journal of gastroenterology : WJG Vol. 15; no. 15; pp. 1816 - 1820
• Main Authors: Zeng, Yun, Liu, Gang, Zhou, Li-Ming
• Format: Journal Article
• Language: English
• Published: United States Department of Pharmacology, West China Medical Center of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China 21.04.2009; Institute of Materia Medica and Department of Pharmacology, North Sichuan Medical College, Nanchong 637%Department of Pharmacology, West China Medical Center of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China; The WJG Press
• Subjects: Animals; Anthraquinones - pharmacology; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Caspase 3 - metabolism; Cell Cycle - drug effects; Cell Line, Tumor - drug effects; Cell Line, Tumor - metabolism; Cell Proliferation - drug effects; Drugs, Chinese Herbal - pharmacology; Humans; Male; Mice; Mice, Nude; MTT法检测; Neoplasm Transplantation; Original; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; SGC; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; 人胃癌细胞株; 实验研究; 抑制作用; 流式细胞仪检测; 诱导细胞凋亡; 逆转录聚合酶链反应; Acetylshikonin; Antitumor effect; SGC-7901 cells; Apoptosis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.1816

AIM： To investigate the inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 and its mechanism. METHODS： MTT assay was used to assess the inhibitory effect of acetylshikonin on proliferation of SGC-7901 cells. Apoptosis-inducing effect was determined by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling with Hoechst staining. Expression of mRNA and protein in Bcl-2 and Bax was analyzed by reverse transcription-polymerase chain reaction and Western blot. Antitumor effect of acetylshikonin on a mouse SGC-7901 model was also determined. RESULTS： Forty-eight hours after treatment with acetylshikonin, MTT assay showed that acetylshikonin inhibited the proliferation of SGC-7901 cells in a dose-dependent manner. The half maximal inhibitory concentration of acetylshikonin to SGC-7901 cells was 0.428 ＋ 0.07 mg/L. Cell shrinkage, nuclear pyknosis and chromatin condensation, which are the characteristics of cell apoptosis, were observed in treated SGC-7901 cells and the percentage of apoptosis increased in a dose-dependent manner. Acetylshikonin down- regulated the expression of Bcl-2 and up-regulated the expression of Bax in the treated SGC-7901 cells compared with the controls. The experiment in vivo showed that 0.5, 1, and 2 mg/kg of acetylshikonin significantly inhibited the growth of tumor in the mouse SGC-7901 model, with an inhibitory rate of 25.00%-55.76%. CONCLUSION： Acetylshikonin inhibits the growth of SGC-7901 cells in vitro and in vivo by inducing cell apoptosis.