SUPPRESSED INCREASE IN BLOOD ENDOTHELIAL PROGENITOR CELL CONTENT AS RESULT OF SINGLE EXHAUSTIVE EXERCISE BOUT IN MALE REVASCULARISED CORONARY ARTERY DISEASE PATIENTS

• Published in: Acta clinica belgica (English ed. Online) Vol. 67; no. 4; pp. 262 - 269
• Main Authors: Rummens, JL, Daniëls, A, Dendale, P, Hensen, K, Hendrikx, M, Berger, J, Koninckx, R, Hansen, D
• Format: Journal Article
• Language: English
• Published: Gent Taylor & Francis 01.07.2012; University Hospital Gent; Acta Clinica Belgica; Taylor & Francis Ltd
• Subjects: Angina pectoris; Angioplasty, Balloon, Coronary; Antigens, CD - blood; Biological and medical sciences; Blood cells; Cardiac patients; Cardiology. Vascular system; Cardiovascular disease; Coronary Artery Bypass; coronary artery disease; Coronary Artery Disease - blood; Coronary Artery Disease - therapy; Coronary heart disease; Endothelial Cells - cytology; endothelial progenitor cells; Exercise; flow cytometry; General aspects; Health aspects; Heart; Heart attacks; Heart rate; Humans; Male; Medical sciences; Middle Aged; Physiological aspects; Stem Cells - cytology; Belgium; Physical exercise; Human; coronary artery disease; Flow cytometry; Endothelial cell; Stem cell; Hematopoietic cell; Cardiovascular disease; Patient; Male; exercise; Increase; Coronary heart disease; Endothelium; Result; Medicine; Content; endothelial progenitor cells; Progenitor cell
• ISSN: 1784-3286; 2295-3337
• DOI: 10.2143/ACB.67.4.2062670

Endothelial progenitor cells (EPCs) significantly affect endothelial repair capacity and, hence, cardiovascular disease incidence. In healthy subjects, blood EPC content increases significantly as result of a single maximal exercise test, hereby stimulating endothelial repair capacity. It remains to be shown whether a single exercise positively affects blood EPCs in revascularised coronary artery disease (CAD) patients. From male revascularised CAD patients (n = 60) and healthy volunteers (n = 25) blood samples were collected before and immediately after a maximal cardiopulmonary exercise test. Blood samples were analyzed by optimised flow cytometry methodology for EPC content (CD34+, CD34+ CD133+, CD34+VEGFR2+, CD34+CD133+VEGFR2+, and CD34+CD133-VEGFR2+ cells) and compared between groups. CFU-Hill colonies were additionally assessed. As a result of a maximal exercise test, blood CD34+, CD34+VEGFR2+ (all EPCs), CD34+CD133+, and CD34+ CD133-VEGFR2+ (mature EPCs) cells increased significantly in CAD patients (p < 0.05), but less than in healthy subjects (p < 0.05, and p = 0.06 for CD34+VEGFR2+). CD34+CD133+VEGFR2+ cells (immature EPCs) did not change as result of exercise (p > 0.05). No changes in CFUHill colonies as result of exercise were observed. This study shows that blood mature EPCs (CD34+CD133- VEGFR2+) increase significantly as result of a single exercise bout in revascularised CAD patients, but with smaller magnitude compared to healthy subjects. Blood immature EPCs (CD34+CD133+VEGFR2+) did not change significantly as result of exercise.