Lethality of cytochalasin B and other compounds isolated from fungus Aspergillus sp. (Trichocomaceae) endophyte of Bauhinia guianensis (Fabaceae)

• Published in: Revista argentina de microbiología Vol. 48; no. 3; pp. 259 - 263
• Main Authors: Feitosa, André de O., Dias, Amanda Cristina S., Ramos, Gisele da C., Bitencourt, Heriberto R., Siqueira, José Edson S., Marinho, Patrícia Santana B., Barison, Andersson, Ocampos, Fernanda M.M., Marinho, Andrey Moacir do R.
• Format: Journal Article
• Language: English
• Published: Argentina Elsevier España, S.L.U 01.07.2016
• Subjects: Acetylation; Animals; Argentina; Artemia - drug effects; Artemia salina; Aspergillus - chemistry; Aspergillus - isolation & purification; Cytochalasin B - chemistry; Cytochalasin B - isolation & purification; Cytochalasin B - toxicity; Cytochalasins - chemistry; Cytochalasins - isolation & purification; Cytochalasins - toxicity; Endophytes - chemistry; Endophytes - isolation & purification; Endophytic fungi; Ergosterol - analogs & derivatives; Ergosterol - chemistry; Ergosterol - isolation & purification; Ergosterol - toxicity; Fabaceae - microbiology; Hongos endófitos; Lethal Dose 50; Metabolitos secundarios; Mevalonic Acid - analogs & derivatives; Mevalonic Acid - chemistry; Mevalonic Acid - isolation & purification; Mevalonic Acid - toxicity; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Secondary metabolites; Spectrometry, Mass, Electrospray Ionization; Structure-Activity Relationship; Metabolitos secundarios; Artemia salina; Secondary metabolites; Endophytic fungi; Hongos endófitos
• ISSN: 0325-7541
• DOI: 10.1016/j.ram.2016.04.002

Endophytic fungi are fungi that colonize internal tissues of plants; several biologically active compounds have been isolated from these fungi. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, this study aimed the isolation and structural identification of ergosterol (1), ergosterol peroxide (2), mevalonolactone (3), cytochalasin B (4) and cytochalasin H (5) from Aspergillus sp. EJC 04, an endophytic fungus from Bauhinia guianensis. The cytochalasin B (4) and the diacetate derivative of cytochalasin B (4a) showed high lethality in the brine shrimp assay. This is the first occurrence of cytochalasins in Amazonian endophytic fungi from B. guianensis. Los hongos endofíticos son hongos que colonizan los tejidos internos de las plantas; varios compuestos biológicamente activos se han aislado a partir de estos hongos. Existen pocos estudios de compuestos aislados de hongos endófitos de plantas amazónicas. Por lo tanto, este estudio tuvo como objetivo el aislamiento y la identificación estructural de ergosterol (1), peróxido de ergosterol (2), mevalonolactona (3), citocalasina B (4) y citocalasina H (5) a partir de Aspergillus spp. EJC 04, un hongo endofítico de Bauhinia guianensis. La citocalasina B (4) y el derivado diacetato de citocalasina B (4a) mostraron una alta letalidad en el ensayo de Artemia salina. Esta es la primera aparición de citocalasinas en hongos endófitos amazónica de B. guianensis.