Oxidative DNA cleavage induced by an iron(III) flavonoid complex: Synthesis, crystal structure and characterization of chlorobis(flavonolato)(methanol) iron(III) complex

• Published in: Journal of inorganic biochemistry Vol. 100; no. 7; pp. 1208 - 1218
• Main Authors: El Amrani, Fátima Ben Allal, Perelló, Lourdes, Real, Jose A., González-Alvarez, Marta, Alzuet, Gloria, Borrás, Joaquín, García-Granda, Santiago, Montejo-Bernardo, José
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2006
• Subjects: Crystallography, X-Ray; DNA - chemistry; DNA - drug effects; Electron Spin Resonance Spectroscopy; Ferric Compounds - chemical synthesis; Ferric Compounds - chemistry; Ferric Compounds - pharmacology; Flavonoid; Iron(III); Models, Molecular; Molecular Structure; Oxidative DNA cleavage; Oxidative Stress; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Iron(III); Flavonoid; Oxidative DNA cleavage
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/j.jinorgbio.2006.01.036

A flavonol iron(III) complex, [Fe(flavonolato) 2Cl(MeOH)], has been prepared. The compound has been characterized by X-ray crystallography, spectroscopy, magnetism and electronic paramagnetic resonance (EPR) at X- and Q-band. The geometrical environment around the metal is best described as rhombic distorted octahedral. This distortion has also been inferred from the magnetic measurements and from the EPR spectra at different temperatures, E/ D(rhombicity parameter) ≈ 0.06. The DNA cleavage activity of the iron(III) complex with and without ascorbate/hydrogen peroxide is reported. Mechanisms of the oxidative cleavage have been proposed when DNA strand scission is performed both with and without ascorbate/hydrogen peroxide activation.