Assessment of exposure to pesticides in rural workers in southern of Minas Gerais, Brazil

• Published in: Environmental toxicology and pharmacology Vol. 55; pp. 99 - 106
• Main Authors: Silvério, Alessandra Cristina Pupin, Machado, Simone Caetani, Azevedo, Luciana, Nogueira, Denismar Alves, de Castro Graciano, Miriam Monteiro, Simões, Juliana Savioli, Viana, André Luiz Machado, Martins, Isarita
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2017; Elsevier Science Ltd
• Subjects: Acetylcholinesterase; Adult; Alkanes - analysis; Alkanes - blood; Alkanes - urine; Bioindicators; Biomarkers; Biomonitoring; Blood - drug effects; Blood - metabolism; Brazil; Cancer; Cell death; Cholinesterase; Cholinesterase activity; Cholinesterases - metabolism; Chromatin; Clinical evaluation; Control methods; Cytome assay; Deoxyribonucleic acid; DNA; DNA Damage; Exposure; Female; Genotoxicity; Human exposure; Humans; Indicator species; Male; Micronuclei; Micronucleus Tests; Middle Aged; Mouth - drug effects; Mouth - metabolism; Mutagenicity Tests; Occupational exposure; Occupational Exposure - adverse effects; Occupational health; Occupational toxicology; Organophosphates; Organophosphates - adverse effects; Pesticides; Pesticides - adverse effects; Phosphates; Rural Population; Toxicity; Urinary dialkyl phosphates; Urine; Urine - chemistry; Brazil; Minas Gerais Brazil; Clinical evaluation; Cholinesterase activity; Occupational toxicology; Urinary dialkyl phosphates; Cytome assay; Organophosphates
• ISSN: 1382-6689; 1872-7077; 1872-7077
• DOI: 10.1016/j.etap.2017.08.013

[Display omitted] •Rural workers exposed to pesticides have risk of developing toxicological effects.•Rural workers exposed to pesticides present susceptibility to developing cancer.•Data showed hazardous exposure to organophosphates. The aim of the study was to assess of occupational exposure to pesticides in rural workers using genotoxicity test, bioindicators and clinical evaluation. Blood, urine and buccal samples from persons, rural workers exposed to a complex mixture of pesticides with organophosphates (n=94) and without organophosphates (n=94) were collected to compare the activities of cholinesterases, the levels of urinary dialkyl phosphates, genotoxicity data, from a cytome assay. Biomarkers were analysed by traditional/published methods Control group consisted of 50 other persons, non- occupationally exposed to pesticides from the city of Alfenas, Minas Gerais, Brazil. All subjects underwent a clinical evaluation. In the group exposed to organophosphates, the activity of acetylcholinesterase, butyrylcholinesterase and total cholinesterase was lower by 63.8%, 12.8%, and 14.8%, respectively, and 92.6% of the group had dialkyl phosphates present in their urine. The cytome assay was used to measure biomarkers of DNA damage (micronuclei and/or elimination of nuclear material by budding), cytokinetic defects (binucleated cells), and proliferative potential (basal cell) and/or cell death (condensed chromatin, karyorrhectic, pyknotic, and karyolytic cells). The group exposed to organophosphates showed significant changes in all these parameters compared to the control group and showed significant changes in budding, condensed chromatin and karyolytic cells compared with the group non-exposed to organophosphates. Data from the clinical evaluation showed significant changes in the central nervous, respiratory and auditory systems. The studied biomarkers are able to distinguish occupational and environmental exposure to pesticides and the data showed hazardous exposure to organophosphates and afforded valuable data to estimate the risk to cancer development.