Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-κB in Acute Transection Spinal Cord Injury in Rats

• Published in: Journal of Korean Neurosurgical Society Vol. 56; no. 5; pp. 375 - 382
• Main Authors: Shrestha, Rajiv Prasad, Qiao, Jian Min, Shen, Fu Guo, Bista, Krishna Bahadur, Zhao, Zhong Nan, Yang, Jianhua
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Neurosurgical Society 01.11.2014; 대한신경외과학회
• Subjects: Laboratory Investigation; 신경외과학; NF-κB; Inflammation; Neuroprotective agent; Spinal cord injury; Mononuclear cells; Cell transplantation
• ISSN: 2005-3711; 1598-7876
• DOI: 10.3340/jkns.2014.56.5.375

To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-κB (NF-κB) in spinal cord injury (SCI) in rats. BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-κB in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction. At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-κB positive cells and level of NF-κB messenger RNA in spinal cord at various time points between the groups. Activated NF-κB immunoreactivity and level of NF-κB mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05). BMMNCs transplantation attenuates the expression of NF-κB in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.