Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function

• Published in: Environmental science and pollution research international Vol. 26; no. 3; pp. 2375 - 2386
• Main Authors: Tremblay-Laganière, Camille, Garneau, Léa, Mauger, Jean-François, Peshdary, Vian, Atlas, Ella, Nikolla, Alyssa Samantha, Chapados, Natalie Ann, Aguer, Céline
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2019; Springer Nature B.V
• Subjects: Animals; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biocompatibility; Biphenyl; Catalase; Catalase - genetics; Catalase - metabolism; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P450; Detoxification; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental Pollutants - toxicity; Environmental science; Exposure; Female; Gene Expression Regulation, Enzymologic - drug effects; Glucose; Glutaredoxin; Glutaredoxins - genetics; Glutaredoxins - metabolism; In vivo methods and tests; Inactivation, Metabolic - drug effects; Inactivation, Metabolic - genetics; Metabolic disorders; Metabolism; Mitochondria; Mitochondria, Muscle - drug effects; Mitochondria, Muscle - metabolism; mRNA; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Oxidative stress; Oxidative Stress - drug effects; Oxygen - metabolism; Oxygen consumption; PCB; Pollutants; Polychlorinated biphenyls; Polychlorinated Biphenyls - toxicity; Rats; Rats, Sprague-Dawley; Reactive oxygen species; Regulators; Research Article; Skeletal muscle; Substrates; Toxicology; Waste Water Technology; Water Management; Water Pollution Control; Electron transport chain; Polychlorinated biphenyls; Oxidative stress; Oxidative phosphorylation; Skeletal muscle metabolism
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-018-3738-8

In the past few years, polychlorinated biphenyls (PCBs), a class of environmental pollutants, have been associated with metabolism dysregulation. Muscle is one of the key regulators of metabolism because of its mass and its important role in terms of glucose consumption and glucose storage. It has been shown that muscle alterations, such as oxidative stress and mitochondrial dysfunction, contribute significantly to the development of metabolic diseases. No study has yet investigated the toxicological effect of PCBs on muscle mitochondrial function and oxidative stress in vivo. The aim of this study was to assess the effect of PCB126 in vivo exposure (single dose of 1.05 μmol/kg) on muscle mitochondrial function and oxidative stress in rats. PCB126-treated rats showed a marked increase in Cyp1a1 mRNA levels in skeletal muscles in association with a 40% reduction in state 3 oxygen consumption rate measured with complex I substrates in permeabilized muscle fibers. Furthermore, PCB126 exposure altered the expression of some enzymes involved in ROS detoxification such as catalase and glutaredoxin 2. Our results highlight for the first time a toxic effect of coplanar PCBs on skeletal muscle mitochondrial function and oxidative stress. This suggests that acute PCB exposure, by affecting muscle metabolism, could contribute to the development of metabolic disorders. Studies are needed to determine if lower-level but longer-term PCB exposure exhibits the same effect.