Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity

• Published in: Carbohydrate polymers Vol. 223; p. 115104
• Main Authors: Zhang, Shihai, He, Fei, Chen, Xia, Ding, Kan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2019
• Subjects: anion exchange chromatography; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; arabinose; bioactive properties; Carbohydrate Conformation; Carcinoma, Pancreatic Ductal - drug therapy; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Cell Line; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; ethanol; fruits; galactose; galacturonic acid; Humans; Lycium - chemistry; Lycium ruthenicum; Lycium ruthenicum Murr; mitogen-activated protein kinase; molecular weight; neoplasm cells; Pancreatic cancer; pancreatic neoplasms; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; pectins; Pectins - chemistry; Pectins - isolation & purification; Pectins - pharmacology; Polysaccharide; protein synthesis; Rhamnogalacturonan I (RG-I); rhamnose; Structure-Activity Relationship; xylose; NaBH4; CH3I; DMSO; FT-IR; Mw; Rha; MTT; COSY; TFA; LO2; DMEM; GSK-3β; FBS; RG-I; CMC; RG-II; GC–MS; HPDE6-C7; HMBC; Xyl; AKT; HPGPC; GalA; ECL; Ara; FAK; Rhamnogalacturonan I (RG-I); PMP; NMR; Gal; XGA; Pancreatic cancer; CLRP; PMAA; DEAE; HSQC; RPMI; Polysaccharide; Lycium ruthenicum Murr

•A polysaccharide, LRP3-S1 was purified and characterized from Lycium ruthenicum.•The main chain of this polysaccharide was alternate 1,4-α-D-GalpA and 1,2-α-L-Rhap.•The side chains of LRP3-S1 contained arabinans and arabinogalactans.•LRP3-S1 inhibited the growth and invasion of pancreatic cancer ce...