Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity

• Published in: Carbohydrate polymers Vol. 223; p. 115104
• Main Authors: Zhang, Shihai, He, Fei, Chen, Xia, Ding, Kan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2019
• Subjects: anion exchange chromatography; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; arabinose; bioactive properties; Carbohydrate Conformation; Carcinoma, Pancreatic Ductal - drug therapy; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Cell Line; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; ethanol; fruits; galactose; galacturonic acid; Humans; Lycium - chemistry; Lycium ruthenicum; Lycium ruthenicum Murr; mitogen-activated protein kinase; molecular weight; neoplasm cells; Pancreatic cancer; pancreatic neoplasms; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; pectins; Pectins - chemistry; Pectins - isolation & purification; Pectins - pharmacology; Polysaccharide; protein synthesis; Rhamnogalacturonan I (RG-I); rhamnose; Structure-Activity Relationship; xylose; NaBH4; CH3I; DMSO; FT-IR; Mw; Rha; MTT; COSY; TFA; LO2; DMEM; GSK-3β; FBS; RG-I; CMC; RG-II; GC–MS; HPDE6-C7; HMBC; Xyl; AKT; HPGPC; GalA; ECL; Ara; FAK; Rhamnogalacturonan I (RG-I); PMP; NMR; Gal; XGA; Pancreatic cancer; CLRP; PMAA; DEAE; HSQC; RPMI; Polysaccharide; Lycium ruthenicum Murr
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115104

•A polysaccharide, LRP3-S1 was purified and characterized from Lycium ruthenicum.•The main chain of this polysaccharide was alternate 1,4-α-D-GalpA and 1,2-α-L-Rhap.•The side chains of LRP3-S1 contained arabinans and arabinogalactans.•LRP3-S1 inhibited the growth and invasion of pancreatic cancer cells.•LRP3-S1 blocked the FAK/AKT/GSK-3β and p38 MAP kinase signaling pathways. Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose™Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Galp, 1,3-linked β-D-Galp, 1,6-linked β-D-Galp, 1,3,6-linked β-D-Galp, 1,5-linked α-L-Araf, 1,3,5-linked α-L-Araf, T-linked α-L-Araf and T-linked β-D-Xylp. Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.