Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus

• Published in: Chinese journal of oceanology and limnology Vol. 27; no. 1; pp. 92 - 99
• Main Author: 陈妍婕 张全启 齐洁 孙业盈 钟其旺 王旭波 王志刚 李朔 李春梅
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.02.2009; Springer Nature B.V; Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China
• Subjects: Complementary DNA; Creatine; Creatine kinase; Differential display; Earth and Environmental Science; Earth Sciences; Eyes; Fragments; Genes; Hybridization; Hybrids; Kareius bicoloratus; Kinases; Larvae; Marine; Marine fishes; Metamorphosis; Migrations; Nucleotide sequence; Oceanography; Paralichthys olivaceus; PCR; PCR技术; Pleuronectiformes; Polymerase chain reaction; Stone; Trypsinogen; Vertebrae; 差异显示; 相关基因; China; INW, Japan; Eye-position; DD-RT-PCR; hybrid; RDA; Kareius bicoloratus; Paralichthys olivaceus
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-009-0092-1

Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis, during which some become sinistral while others dextral. However, the mechanism behinds the eye-position has not been well understood. In this research, hybrids between Japanese flounder（♀） and stone flounder （♂） show mixed eye-location in both dextral type and sinistral type, and thus become good samples for studying the eye-migration, mRNAs from pro-metamorphosis sinistral and dextral hybrids larvae were screened with classical differential display RT-PCR （DD-RT-PCR） and representational difference analysis of cDNA （cDNA-RDA）; 30 and 47 putative fragments were isolated, respectively. The cDNA fragments of creatine kinase and trypsinogen 2 precursor genes isolated by cDNA-RDA exhibited eye-position expression patterns during metamorphosis. However, none of the fragments was proved to be related to flatfishes＇ eye-position specifically. Therefore, further studies and more sensitive gene isolated methods are needed to solve the problems.