Comparative Proteomic Profile of the Human Placenta in Normal and Fetal Growth Restriction Subjects

Background: Fetal growth restriction (FGR) is the main cause of intrauterine fetal death and the second leading cause of death in the neonatal period. A large body of evidence suggests that FGR may be associated with the placenta, although its etiology and pathogenesis remain to be fully elucidated....

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Published inCellular physiology and biochemistry Vol. 34; no. 5; pp. 1701 - 1710
Main Authors Miao, Zhijing, Chen, Min, Wu, Hong, Ding, Hongjuan, Shi, Zhonghua
Format Journal Article
LanguageEnglish
Published Basel, Switzerland Cell Physiol Biochem Press GmbH & Co KG 01.01.2014
Subjects
Adult
Apoptosis
Case-Control Studies
Female
Fetal Development - physiology
Fetal growth restriction
Fetal Growth Retardation - metabolism
Humans
Original Paper
Oxidative stress
Placenta
Placenta - metabolism
Pregnancy
Proteome - metabolism
Proteomics - methods
TMT
Oxidative stress
TMT
Placenta
Fetal growth restriction
Apoptosis
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Summary:Background: Fetal growth restriction (FGR) is the main cause of intrauterine fetal death and the second leading cause of death in the neonatal period. A large body of evidence suggests that FGR may be associated with the placenta, although its etiology and pathogenesis remain to be fully elucidated. Methods and Results: To better understand the molecular mechanisms underlying the pathological development of the placenta in FGR, we used tandem mass tags (TMTs) to construct a large-scale comparative proteomic profile of human placentas from normal and FGR pregnancies. A total of 1,198 kinds of proteins were identified in the control and FGR placentas, of which 95 were differentially expressed between two groups. Ingenuity Pathway Analysis (IPA) was used to organize these differentially expressed proteins into networks of interacting proteins and to identify the modules of functionally related proteins. Western blotting was used to verify the expression patterns of several randomly selected proteins. Conclusion: The placentas of women with FGR displayed significant proteome differences compared with normal pregnancy. The results indicate that a variety of mechanisms and proteins may contribute to the development of FGR. Further studies and validations are required to elucidate the exact roles of these proteins in FGR pathogenesis.
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ISSN:1015-8987
1421-9778
1421-9778
DOI:10.1159/000366371