Mechanism of the inhibitory effect of pranoprofen on sodium urate crystal-induced inflammation

• Published in: Nihon yakurigaku zasshi Vol. 89; no. 3; p. 139
• Main Authors: Aratani, H, Iwahisa, Y, Imayoshi, T, Nagaoka, Y, Terasawa, M, Maruyama, Y
• Format: Journal Article
• Language: Japanese
• Published: Japan 1987
• Subjects: Animals; Benzopyrans - pharmacology; Chemotactic Factors - biosynthesis; Chemotaxis, Leukocyte - drug effects; Colchicine - pharmacology; Dinoprostone; Female; Gout Suppressants - pharmacology; Guinea Pigs; Indomethacin - pharmacology; Interleukin-8; Male; Neutrophils - drug effects; Phagocytosis - drug effects; Phenylbutazone - pharmacology; Propionates - pharmacology; Prostaglandins E - biosynthesis; Rats; Rats, Inbred Strains
• ISSN: 0015-5691
• DOI: 10.1254/fpj.89.139

The mechanism of the inhibitory effect of pranoprofen on sodium urate crystal-induced inflammation was investigated with several inflammatory parameters using leucocytes stimulated with sodium urate crystals in vitro. At 10(-5) M, pranoprofen tended to inhibit the production of chemotactic factor in guinea pig polymorphonuclear leucocytes (PMNL) stimulated with sodium urate crystals and at 10(-4) M, significantly inhibited it. At 10(-3) M and 10(-4) M, it potentiated the chemotaxis of guinea pig PMNL. Furthermore, it inhibited the production of superoxide anion (O2-) in guinea pig PMNL stimulated with sodium urate crystals, with an IC50 value of 5.0 X 10(-4) M, comparable to that of indomethacin. At 10(-3) M, it inhibited the release of beta-glucuronidase stimulated by sodium urate crystals. From doses as low as 10(-6) M, it inhibited dose-dependently the production of PGE2-like substance by the phagocytosis of sodium urate crystals by rat peritoneal leucocytes, with an IC50 value of 7.5 X 10(-6) M. These results suggest that in inhibiting the production of PGE2 stimulated by sodium urate crystals, pranoprofen shows an inhibitory effect on sodium urate crystal-induced inflammation.