Measuring levels of biogenic amines and their metabolites in rat brain tissue using high-performance liquid chromatography with photodiode array detection

• Published in: Archives of pharmacal research Vol. 39; no. 1; pp. 59 - 65
• Main Authors: Gu, Min-Jung, Jeon, Ji-Hyun, Oh, Myung Sook, Hong, Seon-Pyo
• Format: Journal Article
• Language: English
• Published: Seoul Pharmaceutical Society of Korea 2016; 대한약학회
• Subjects: acetonitrile; Animals; Biogenic Amines - analysis; Biogenic Amines - metabolism; brain; Brain - metabolism; Brain Chemistry; Chromatography, High Pressure Liquid - methods; correlation; derivatization; dopamine; Dopamine - analysis; Dopamine - metabolism; high performance liquid chromatography; homovanillic acid; Male; Medicine; metabolites; perchloric acid; Pharmacology/Toxicology; Pharmacy; Rats; Rats, Sprague-Dawley; Research Article; serotonin; Serotonin - analysis; Serotonin - metabolism; 약학; Direct method; Simultaneous determination; Dopamine; Serotonin; HPLC–PDA; Brain tissue
• ISSN: 0253-6269; 1976-3786; 1976-3786
• DOI: 10.1007/s12272-015-0661-0

We developed a method to detect biogenic amines and their metabolites in rat brain tissue using simultaneous high-performance liquid chromatography and a photodiode array detection. Measurements were made using a Hypersil Gold C-18 column (250 × 2.1 mm, 5 µm). The mobile phase was 5 mM perchloric acid containing 5 % acetonitrile. The correlation coefficient was 0.9995–0.9999. LODs (S/N = 3) and LOQs (S/N = 10) were as follows: dopamine 0.4 and 1.3 pg, 3, 4-dihydroxyphenylacetic acid 8.4 and 28.0 pg, serotonin 0.4 and 1.3 pg, 5-hydroxyindolacetic acid 3.4 and 11.3 pg, and homovanillic acid 8.4 and 28.0 pg. This method does not require derivatization steps, and is more sensitive than the widely used HPLC–UV method.