Comparison of proteomic and metabolomic profiles of mutants of the mitochondrial respiratory chain in Caenorhabditis elegans

Single-gene mutations that disrupt mitochondrial respiratory chain function in Caenorhabditis elegans change patterns of protein expression and metabolites. Our goal was to develop useful molecular fingerprints employing adaptable techniques to recognize mitochondrial defects in the electron transpo...

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Published inMitochondrion Vol. 20; pp. 95 - 102
Main Authors Morgan, P G, Higdon, R, Kolker, N, Bauman, A T, Ilkayeva, O, Newgard, C B, Kolker, E, Steele, L M, Sedensky, M M
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2015
Subjects
Animals
Caenorhabditis elegans
Caenorhabditis elegans - chemistry
Caenorhabditis elegans Proteins - analysis
Caenorhabditis elegans Proteins - genetics
Electron Transport Chain Complex Proteins - genetics
Metabolome
Mitochondria - enzymology
Mitochondrial Proteins - genetics
Mutation
Nematoda
Proteome - analysis
Metabolomics
Genetics
C. elegans
Mitochondria
Gene expression
Proteomics
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Summary:Single-gene mutations that disrupt mitochondrial respiratory chain function in Caenorhabditis elegans change patterns of protein expression and metabolites. Our goal was to develop useful molecular fingerprints employing adaptable techniques to recognize mitochondrial defects in the electron transport chain. We analyzed mutations affecting complex I, complex II, or ubiquinone synthesis and discovered overarching patterns in the response of C. elegans to mitochondrial dysfunction across all of the mutations studied. These patterns are in KEGG pathways conserved from C. elegans to mammals, verifying that the nematode can serve as a model for mammalian disease. In addition, specific differences exist between mutants that may be useful in diagnosing specific mitochondrial diseases in patients.
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These authors contributed equally to this manuscript.
ISSN:1567-7249
1872-8278
DOI:10.1016/j.mito.2014.12.004