Image analysis of hyphal morphogenesis in Saprolegniaceae (Oomycetes)

• Published in: Fungal genetics and biology Vol. 41; no. 3; pp. 293 - 307
• Main Authors: Diéguez-Uribeondo, Javier, Gierz, Gerhard, Bartnicki-Garcı́a, Salomon
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2004
• Subjects: Aphanomyces; Hyphae - cytology; Hyphae - growth & development; Leptolegnia; Mathematics; Models, Theoretical; Morphogenesis; Oomycetes - cytology; Oomycetes - growth & development; Saprolegnia parasitica; Saprolegniaceae; Species Specificity
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2003.10.012

Because of their wide range of apical morphology, several members of saprolegniaceous fungi (Oomycetes) were chosen to examine concordance with the vesicle supply center (VSC) model of hyphal morphogenesis. Two computer routines were devised to measure diameter changes over long stretches of hyphae and to test compatibility with the theoretical hyphoid shape, y= xcot( xV/ N). In all four genera examined, the apex followed closely the contour described by the hyphoid equation; divergences became evident in the subapex. The hyphae of Saprolegnia parasitica showed maximum concordance with the VSC model, i.e., their profile matched a hyphoid curve from the apex to the entire length of the mature hyphal tube. In Aphanomyces and Leptolegnia, growth in the subapical region subsided becoming less than that specified by the hyphoid equation. In Achlya bisexualis, the reverse was true, the subapical region expanded beyond that specified by the hyphoid equation. The two divergent subapical tendencies gave the hyphal tips a cylindroid or conoid appearance, respectively. Since the hyphal apex of all four species conformed to the curvature dictated by the hyphoid equation, we concluded that a basic VSC mechanism operates in all of these oomycetous fungi. Accordingly, we suggest that the shape of an oomycetous hypha is generated by a VSC-driven gradient of wall formation, which is subject to additional modification in the subapex to produce a range of hyphal tip morphologies. The mathematical basis for generating a conoid hyphal tip by elongating the VSC is described in Appendix A. Index descriptors: Conoid tips; Growth; Hyphae; Hyphal diameter; Hyphoid model; Morphogenesis; Morphology; Oomycetes; VSC