Thrombin Induces Production of Growth Factors from Aortic Smooth Muscle Cells

• Published in: The Journal of surgical research Vol. 82; no. 1; pp. 61 - 66
• Main Authors: Cucina, Alessandra, Borrelli, Valeria, Di Carlo, Anna, Pagliei, Sabrina, Corvino, Valentina, Santoro-D'Angelo, Luciana, Cavallaro, Antonino, Sterpetti, Antonio V.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.1999; Elsevier
• Subjects: Animals; Antibodies, Monoclonal; Aorta, Thoracic - cytology; Aorta, Thoracic - drug effects; Aorta, Thoracic - metabolism; Arteries - injuries; Arteries - pathology; Arteries - surgery; bFGF; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cattle; Cell Division - drug effects; Cells, Cultured; Culture Media, Conditioned; Diseases of the aorta; DNA - biosynthesis; Fibroblast Growth Factor 2 - biosynthesis; Fibroblast Growth Factor 2 - immunology; growth factors; Humans; Hyperplasia; Kinetics; Medical sciences; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; PDGF; Platelet-Derived Growth Factor - biosynthesis; Platelet-Derived Growth Factor - immunology; smooth muscle cell; thrombin; Thrombin - pharmacology; smooth muscle cell; thrombin; growth factors; PDGF; bFGF; Cell culture; Hemostatic; Hyperplasia; Cardiovascular disease; Smooth muscle; Thrombin; Basic fibroblast growth factor; Arterial disease; Vascular disease; Production; Intima; Aorta; Complication; Ungulata; Platelet derived growth factor; Reconstruction; Serine endopeptidases; Bovine; Pathophysiology; Enzyme; Cytokine; Plastic surgery; Experimental study; Peptidases; Vertebrata; Mammalia; Polypeptide; Animal; Hydrolases; Artiodactyla; Aortic disease; Growth factor
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1006/jsre.1998.5514

Background.Myointimal hyperplasia is a common complication of arterial recontructive surgery. The serine protease thrombin has a major role in vessel wall healing and eventual myointimal hyperplasia formation. The aim of this study was to determine the effect of thrombin on the production of PDGF AA and bFGF by arterial smooth muscle cells. Materials and methods.Bovine smooth muscle cells were stimulated with thrombin in a serum-free culture. The release of PDGF AA and bFGF was assessed by ELISA. The effect of thrombin on the proliferation of confluent monolayers of bovine smooth muscle cells was determined by tritiated thymidine uptake. Results.Smooth muscle cells stimulated with thrombin released more PDGF AA (P< 0.001) and bFGF (P< 0.001) than the control. Addition of anti-PDGF AA and anti-bFGF antibodies to the medium of smooth muscle cell cultures neutralized the mitogenic effect of thrombin (P< 0.001). Conclusions.The findings of our study suggest that thrombin may lead to myointimal hyperplasia formation through induction of PDGF and bFGF production by smooth muscle cells.